Biological Function and Mechanism of STC2 in TU686 Cells
Objective Laryngeal squamous cell carcinoma(LSCC)is a malignant tumor of the head and neck,the incidence of which is increasing worldwide.In the early stage of this study,stanniocalcin 2(STC2)was significantly upregulated in LSCC com-pared with paracancer tissues,but its regulatory effect on tumor cells in LSCC remains to be confirmed.The aim of this study is to e-valuate the biological function of STC2 in LSCC and its possible mechanism of action.Methods This study included 43 patients with LSCC and 4 healthy volunteers.The expression of STC2 in LSCC tumors and adjacent tissues was detected by tissue microarray chip IHC.The expression of STC2 was detected by Western Blot analysis of human immortalized oropharyngeal epithelial cells NP69 and multiple laryngeal carcinoma cells.Lentiviral vector was used to construct TU686 cells with STC2 knockdown and lentivirus-infected TU686 cells were prepared.The effect of STC2 on the proliferation function of laryngeal cancer cell lines was detected by CCK-8 and clonal formation assay.Flow cytometry,scratch assay and transwell migration assay were used to evaluate the effects of STC2 on apop-tosis and migration of laryngeal carcinoma cells.Results IHC confirmed that STC2 was highly expressed in LSCC tumor tissues com-pared with paracancer tissues.Compared with normal oropharyngeal epithelial cells,STC2 was highly expressed in laryngeal carcinoma cells.The high expression of STC2 is associated with T infiltration and clinical stage in LSCC patients.The results of TU686 cell func-tion experiment after STC2 knockdown showed that STC2 knockdown could reduce the proliferation and migration ability of laryngeal carcinoma cells and induce cell apoptosis.Conclusions STC2 can promote LSCC and affect the progression of LSCC,and its mecha-nism may be related to T lymphocyte infiltration.
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