Effect of Allicin on AGEs/PKC/p66shc Pathway in Kidney of Type 2 Diabetes Rats
Objective To establish A type 2 diabetic nephropathy rat model by intrabitoneal injection of streptozotocin(strepto-zotocin,STZ),and to investigate the protective effect of allicin on the kidney of type 2 diabetic rats and its mechanism.Methods 30 SD male rats were divided into 5 groups:normal control group(Control),diabetic nephropathy model group(Model),low dose allicin protection group(DT10),medium dose allicin protection group(DT20)and high dose allicin protection group(DT40).The levels of creatinine,microalbumin,malondialdehyde and the activity of superoxide dismutase were detected by the kit.the morpho-logical changes of kidney were observed by HE and PAS staining.Western Blot was used to detect the expression of related proteins.Results Compared with model group,the levels of creatinine,microalbumin and malondialdehyde in allicin protected group were sig-nificantly decreased(P<0.05),and the activity of superoxide dismutase was significantly increased(P<0.05).The results of HE and PAS staining showed that the microstructure of renal tissue was improved and the glycogen deposits in renal interstitial were re-duced.Western Blot results showed that compared with model group,the levels of AGEs,P-PKC and p66shc in allicin protected group were significantly decreased(P<0.05).Conclusion Allicin has a protective effect on the kidney of diabetic rats,and the mechanism of this protective effect may be related to the regulation of AGEs/PKC/p66shc signaling pathway.
diabetic nephropathycarnosineadvanced glycation end productsprotein kinase C
NETL
NSTL
万方数据
