Mechanism Observation of CX3CL1 Promoting Doxorubicin/Paclitaxel Chemoresistance in Triple Negative Breast Cancer
Objective To explore the mechanism of CX3CL1 gene promoting drug resistance to doxorubicin and paclitaxel in tri-ple negative breast cancer.Methods CX3CL1 gene overexpression lentiviral vector was constructed,CX3CL1 expression in breast cancer cells was detected by Western Blot,gene expression in cells was detected by Q-PCR,cell viability was detected by CCK-8 as-say,Edu was used to detect cell proliferation,apoptosis was detected by flow cytometry with Annexin V-FITC/PI staining,Transwell was used to detect cell mobility,and CX3CL1 expression in intracellular and culture supernatant was detected by Elisa kit.The ex-pression of CX3CLI in triple-negative breast cancer was analyzed by SPSS 19.0 statistical software and Pearson Chi-square test.The cumulative survival rate was calculated by Kaplan-Meier method.Results The constructed CX3CL1 overexpression lentivirus infected MDA-MB-231 cell vector showed that CX3CL1 has the effects of promoting cell viability,promoting cell proliferation,inhibiting cell apoptosis,and promoting cell migration.Through the binding of human monocytic leukemia cells,it was found that CX3CL1 can in-duce macrophage polarization towards M2 type and promote cell migration.Conclusion CX3CL1 can reduce the sensitivity of breast cancer cells to doxorubicin/paclitaxel chemotherapy drugs by promoting the polarization of macrophages to M2 type,inhibiting cell ap-optosis and promoting cell migration,thus leading to the mechanism of drug resistance.
CX3CL1triple negative breast cancerchemotherapy drugsdrug resistance mechanism
NETL
NSTL
万方数据
