Functional Selenium-doped Silica Nanoparticles Loaded with Resveratrol for the Treatment of Spinal Cord Injury
Objective To prepare a nano drug delivery system(PM-MSe-Res)and establish a mouse spinal cord injury(SCI)model to investigate the therapeutic role of PM-MSe-Res in spinal cord injury.Methods C57BL/6J mice weighing 17-25 g were used as experimental subjects,which were randomly divided into 5 groups,namely,the sham-operated group(Sham),the spinal cord injury group(SCI),the resveratrol group(Res),the plasma-modified diselenium-bonded doped mesoporous silica group(PM-MSe)and the plasma-modified diselenium-bonded doped mesoporous silica carrying resveratrol group(PM-MSe-Res).The cumulative release rate of Res was measured by drug release assay.CCK-8 assays were used to test the biosafety of PM-MSe and MSe.The level of oxidative stress in damaged tissues of different treatment groups was detected by glutathione(GSH),superoxide dismutase(SOD)and malondialdehyde(MDA)kits.Finally,immunofluorescence assay was used to verify that PM-MSe-Res exerts anti-inflammatory effects in RAW264.7 cells.Results Res could be rapidly released from PM-MSe,and the biosafety and targeting of MSe were improved after PM modification.PM-MSe-Res could scavenge ROS at the site of injury to exert anti-inflammatory effects.Conclusion PM-MSe can help Res cross the blood-spinal cord barrier and target to the site of injury to exert anti-oxidative and anti-inflammatory effects.
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