Metagenomics Analysis on the Pathogens Carried by Intercepted Cockroaches Imported at Jinan Port
Objective We explored to provide support for the prevention and control of cockroachborne diseases at Jinan port by metagenomics analysis.Methods We selected 623 cockroaches as the research subjects,inter-cepted and submitted for inspection in the jurisdiction of Jinan Customs from 2022 to June 2023.We made body surface samples and tissue samples for every 40-50 cockroaches in a group.We conducted multiple PCR amplifica-tion on samples of Vibrio cholerae,Salmonella,Shigella,and Norovirus,and monitored and analyzed cockroach-borne pathogens through metagenomic sequencing.Results Various bacteria such as cockroach rod-shaped bacte-ria,Escherichia coli,Enterococcus,Acinetobacter,Weisseria,Proteobacterium,Klebsiella,etc.were detected by metagenomic analysis in 15 groups of cockroach samples.Conclusion Pathogen metagenomic analysis can detect multiple pathogens carried by cockroaches,and custom ports need to strengthen cockroach monitoring and timely in-terception.
万方数据
维普
