An Investigation of Bartonella Carried by Rodents at Alashankou Port on China-Kazakhstan Border in 2022
Objective To study and examine the genetic homology and phylogenetic evolution of the rats at Alsshankou port on the China-Kazakhstan border to comprehend the Bartonella carrying status to give the Customs a scientific foundation on which to conduct surveillance and research into diseases transmitted by rats at ports.Methods Eight monitoring zones were chosen in urban areas,suburban regions,fields,and entry and departure routes at Alsshankou Port from March to November 2022.Rodents were caught using a nighttime trapping tech-nique.They were then categorized,recognized,and dissected.The Bartonella citrate synthase gene(gltA)was am-plified using the polymerase chain reaction(PCR)method,and the positive products were sequenced before homol-ogy and genetic evolution tree analysis.Liver tissue was taken aseptically for nucleic acid extraction.Results A to-tal of 101 rodents,representing 5 species,5 genera,2 families,and 1 order were caught.Rhombomys opimus ac-counted for the majority of the captures(66.34％),with 67 individuals,followed by Meriones meridianus,Cricetulus migratorius,Rattus norvegicus,and Mus musculus.PCR was used to identify gltA gene nucleic acid in 101 mice liver samples and 7 gerbils,with a detection rate of 6.93％.With the exception of alsk2022092302,sequencing and genetic evolution analyses revealed that the sequences were comparable.Its 95.56％to 100％homology with six other Bartonella gltA genes(AB444984.1,MG887832.1,KT327028.1,KT327032.1,MH618792.1,and JN402992.1)found in GeneBank suggests that the gerbil population in the Alashankou port area may contain a variety of Barton-ella molecular species.Conclusion The investigation revealed the presence of Bartonella infection in rodents,par-ticularly gerbils in the Alashankou port region to prevent Bartonella from being introduced or transmitted through border ports.Monitoring,prevention,and control must be strengthened.
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