苹果蠹蛾颗粒体病毒(Cydia pomonella granulovirus,CpGV)是控制苹果蠹蛾Cydia pomonella(L.)种群的重要生防因子.商业化产品病毒株单一,抗性的快速产生会降低CpGV的防效,高毒力或与之具有遗传差异的新病毒株可用于延缓病毒抗性的产生或发展,对苹果蠹蛾的控制策略具有重要作用.本研究从中国酒泉地区苹果园中的苹果蠹蛾野生种群及其实验室种群发病虫体中分离获得一株致病病毒,通过幼虫发病特征、病毒包涵体的形态学及分子生物学方法共同证实该病毒株为CpGV,且与CpGV-M相似,将其命名为CpGV-CJ01.其幼虫的发病体征具有CpGV感染的典型症状,包涵体的电镜形态学观察结果符合CpGV结构特征,利用分子生物学方法克隆得到Granulin基因和凋亡抑制蛋白基因(inhibitor of apoptosis protein,iap)均与CpGV-M具有99％以上的相似性,但是对于毒力的准确定量需要做进一步研究.我国新病毒株的发现为我国开展苹果蠹蛾颗粒体病毒的生物学防治创造了先决条件,并可作为延缓世界范围内CpGV抗性的候选菌株.
Characterization of the Cydia pomonella granulovirus CpGV-CJO1 from northwest China
The Cydia pomonella granulovirus ( CpGV) is an effective and selective biological control agent for C. pomonella. Currently most CpGV products are based only on the CpGV-M isolate. Novel isolates could make a great contribution to C. pomonella control, for example, isolated strains with high toxicity that are genetically different to CpGV-M could be used to prevent, or delay, the development of resistance to CpGV. This study isolated a novel CpGV isolate from a wild C. pomonella population in Gansu province and investigated the characteristics of its descendent lab population, including characteristics of infected larvae, observation of CpGV occlusion bodies and molecular biological techniques. The results indicate that this isolate, named CpGV-CJ01, belongs to the CpGV family and is similar to CpGV-M. The symptoms of larvae infected by CpGV-CJOl are similar to the typical symptoms reported for CpGV and the morphological characteristics of the occlusion bodies were confirmed by electron microscopy. The CpGV-CJ01 Granulin gene and the inhibitor of apoptosis protein ( iap) gene were cloned; both shared over 99% similarity with CpGV-M. Further study of the virulence of the new strain is required. The isolation of novel CpGV strains was a prerequisite for further biological control based on CpGV in China. The CpGV-CJ01 strain could provide the key to managing resistance to CpGV.
Cydia pomonellaCpGVisolationsymptom of infected larvaelectron micrographsgene clone
NETL
NSTL
万方数据
维普
