Amplified ribosomal DNA restriction analysis and identification of intestinal aerobic bacteria from 4th instar larva of Dendrolimu kikuchii
11 strains of aerobic bacteria were isolated from the intestine of Dendrolimu kikuchii Matsumura using the plating method. Bacterial genomic DNA was used as templates. A new method of analyzing the genetic diversity of intestinal aerobic bacteria was established using the PCR technique ( primer27f, 1492r) and ARDRA patterns, which used enzyme digestion (Hae Ⅲ and Hind Ⅲ , Hinf Ⅰ and Taq Ⅰ ) of cloned 16S rDNA gene sequences. The results show that 11 strains could be divided into 6 groups at a similarity level of 84% . The results prove that Dendrolimu kikuchii has a genetically diverse, aerobic, intestinal bacterial fauna. Analysis of full-length 16S rDNA sequences from representative strains of the identified bacterial groups showed that these strains belong to Klebsiella sp. (4N05, 4N08 and 4N11) , Lysinibacillus sp. (4N02) , Gamma proteobacterium (4N03) , Bacillus subtilis strain (4N09) , Brevibacillus sp. (4N04, 4N06, 4N07, 4N01 and 4N10). This study provides a theoretical basis for the microbiological control of Dendrolimu kikuchii and enriches the microbial resources library.
NETL
NSTL
万方数据
维普
