Genetic diversity of different geographical populations of Chilo suppressalis in China as determined by AFLP
DNA molecular markers produced by amplified fragment length polymorphism (AFLP) were used to detect genetic variation in 13 geographical populations of Chilo suppressalis (Walker) collected from ll Chinese provinces.A total of 445 distinct alleles were amplified by three AFLP primer pairs from genomic DNA,of which 386 (86.8％) were found to be polymorphic.The coefficient of gene differentiation between populations was 0.74.Genetic identity between the 13 populations was greater than 0.67.Cluster analysis showed that all the tested populations could be grouped into 4clades.The northern Huai River plain clade was comprised of the GY,FN and FNing populations,the east-south region clade of the YX and MH populations,and the Jing-Xiang-Chuang plain clade of the JZ,SY and DY populations.This classification is credible in that the populations within each of the clades share similar climatic conditions and proximity.However,a single population with a relatively long genetic distance to all other populations probably belongs to a random clade.Investigations of genetic diversity between populations should use as many widely dispersed populations as possible.
NETL
NSTL
万方数据
维普
