【目的】刺桐姬小蜂Quadrastichus erythrinae Kim体型小，传统的形态学鉴定方法难以快速准确识别。【方法】本研究测定了刺桐姬小蜂的rDNA ITS1和ITS2序列，根据18S rDNA 部分序列，利用MEGA的最大相似法（Maximum Likehood）构建系统发育树。根据刺桐姬小蜂ITS1和ITS2序列设计了特异引物，应用特异引物对单只刺桐姬小蜂进行PCR扩增，可稳定地扩增出明显的目的DNA条带。【结果】研究表明，基于 ITS 基因的 DNA 条形码技术可以用于刺桐姬小蜂的快速准确鉴定。【结论】因此，采用ITS1和ITS2区的特异性引物可对刺桐姬小蜂进行快速分子鉴定。
Application of DNA barcoding technology to the identification of Quadrastichus erythrinae Kim (Hymenoptera:Eulophidae)
Objectives] Quadrastichus erythrinae Kim (Hymenoptera:Eulophidae) is too small to be identified quickly and accurately by traditional morphological methods. [Methods] The complete sequences of the rDNA ITS1 and ITS2 genes were determined for Q. erythrinae. A phylogenetic tree was established on the basis of variation in 18SrDNA part sequences using MEGA Maximum Likelihood. Species-specific primers of Q. erythrinae were designed based on the rDNA ITS1 and ITS2 sequences. The results of PCR amplification of rDNA ITS1 and ITS2 indicate that these species-specific primers could reliably identify this species. [Results] The results suggest that the species-specific primers developed produce clear, unique and reproducible target DNA bands from individual adult wasps. Molecular identification of Q. erythrinae using species-specific primers from the rDNA ITS1 and ITS2 region is therefore feasible. [Conclusion] This study identified a possible avenue for the molecular identification of Q. erythrinae.
NETL
NSTL
万方数据
维普
