RNAi-mediated mediating of P-Hsp70 gene expression affects Propylaea quatuordecimpunctata longevity and fecundity under different temperatures
[Aim]This study aims to investigate the effects of heat shock protein P-Hsp70 gene expression on the longevity and fecundity of adult 14-spotted ladybugs,Propylaea quatuordecimpunctata,under different temperatures.[Methods]Microinjection delivery of dsRNA was used to interfere with the P-Hsp70 gene of adult P.quatuordecimpunctata.qRT-PCR was used to calculate the interference efficiency of the P-Hsp70 gene.The adults were then exposed to different temperatures(32,35,and 38 ℃)to determine the effect of temperature on their survival and reproduction following P-Hsp70 gene interference.[Results]Our results demonstrate that microinjection delivery of dsRNA can effectively inhibit the expression of the P-Hsp70 gene in adult P.quatuordecimpunctata.The survival rate,longevity,and fecundity of adult P.quatuordecimpunctata treated with microinjection ds-P-Hsp70 were significantly lower than the control group(without injection of dsRNA)under all temperatures.At 32,35,and 38 ℃,the survival rate of adults decreased by 15.5％,16.7％,and 56.8％,respectively.Additionally,the average adult longevity was reduced by 40.6％,51.2％,and 65.8％,respectively.At 32 and 35 ℃,the fecundity of adults decreased by 37.5％and 100.0％,respectively.At 38 ℃,the adults failed to lay eggs with or without dsRNA.[Conclusion]Our findings indicate that the heat shock protein P-Hsp70 gene plays a crucial role in high temperature stress resistance in adult P.quatuordecimpunctata.
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