丝殊角萤叶甲的线粒体基因组测序及系统发育分析
Mitochondrial genome sequencing of the Agetocera filicornis(Laboissiere,1927)and phylogenetic analysis
林兴雨 1席玉强 1尹新明 1宋南1
作者信息
- 1. 河南农业大学植物保护学院,河南绿色害虫防治国际实验室,河南害虫生物防治工程实验室,郑州 450002
- 折叠
摘要
[目的]为探究丝殊角萤叶甲Agetocera filicornis(Laboissiere,1927)线粒体基因组结构特征及叶甲亚科的系统发育关系.[方法]利用高通量测序方法测定丝殊角萤叶甲线粒体基因组序列.选择叶甲亚科Chrysomelinae和萤叶甲亚科Galerucinae的128个物种作为内群,选择肖叶甲亚科Eumolpinae的3个物种作为外群,利用最大似然法和贝叶斯法重建叶甲亚科的系统发育关系.[结果]丝殊角萤叶甲的线粒体基因组全长为15 814bp,包含37个基因(13个蛋白质编码基因、2个核糖体RNA基因和22个转运RNA基因)和一段非编码控制区.其线粒体基因组AT含量丰富(76.6％),并且AT偏斜为正值(0.053),GC偏斜为负值(-0.252).不同的系统发育分析方法构建的系统发育关系支持萤叶甲亚科为单系群,叶甲亚科为非单系群.[结论]本研究首次获得了丝殊角萤叶甲的线粒体基因组全序列.构建了叶甲亚科的系统发育关系,为更好地理解叶甲亚科、萤叶甲亚科和丝殊角萤叶甲的系统发育提供线粒体基因组数据.
Abstract
[Aim]To explore the structural characteristics of the mitochondrial genome of Agetocera filicornis(Laboissiere,1927),and the phylogenetic relationships within the Chrysomelinae subfamily.[Methods]The mitochondrial genome sequence of A.filicornis was determined using high-throughput sequencing methods.We selected 128 species from the subfamilies Chrysomelinae and Galerucinae as ingroups,and three species from the subfamily Eumolpinae as outgroups.The phylogenetic relationships within the Chrysomelinae Subfamily were reconstructed using maximum likelihood and Bayesian methods.[Results]The mitochondrial genome of A.filicornis is 15 814 bp in length,containing 37 genes(13 protein-coding genes,2 ribosomal RNA genes,and 22 transfer RNA genes)and a non-coding control region.The mitochondrial genome has a high AT content(76.6％)with a positive AT-skew(0.053)and a negative GC-skew(-0.252).Different phylogenetic analysis methods support Galerucinae as a monophyletic group and Chrysomelinae as a non-monophyletic group.[Conclusion]This study reports,for the first time,the complete mitochondrial genome of A.filicornis.By constructing the phylogenetic relationships within Chrysomelinae,we provide new mitochondrial genomic data that will contribute to a better understanding of the phylogeny between Chrysomelinae,Galerucinae,and A.filicornis.
关键词
鞘翅目/叶甲亚科/萤叶甲亚科/丝殊角萤叶甲/线粒体基因组/系统发育Key words
Coleoptera/Chrysomelinae/Galerucinae/Agetocera filicornis/mitochondrial genome/phylogeny引用本文复制引用
基金项目
国家自然科学基金(U1904104)
现代农业产业技术体系建设项目(CARS-27)
河南省自然科学基金(232300420010)
出版年
2024
NETL
NSTL
万方数据