首页|两种寄主植物对淡足侧沟茧蜂的引诱作用及挥发物成分分析

两种寄主植物对淡足侧沟茧蜂的引诱作用及挥发物成分分析

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[目的]为了更好的了解寄主植物和甜菜夜蛾Spodoptera exigua幼虫对淡足侧沟茧蜂Microplitis pallidipes趋向行为的影响.[方法]本研究选用玉米和苋菜两种寄主植物,采用"Y"型嗅觉仪测定了淡足侧沟茧蜂对正常植物和幼虫-植物复合体的行为反应,并对其释放的挥发性物质进行了 GC-MS分析与鉴定.[结果]正常的玉米和苋菜对淡足侧沟茧蜂无明显引诱作用,而幼虫-玉米复合体和幼虫-苋菜复合体均有显著的吸引作用,且幼虫-玉米复合体对该寄生蜂的引诱作用更强.GC-MS检测结果显示在正常玉米中含有22种化合物,主要为烷烃和芳香烃类化合物;幼虫-玉米复合体中有31种化合物,以烷烃和醇类化合物为主;在正常苋菜检测到的16种化合物中,烷烃类化合物含量最高,约占54.75%;而幼虫-苋菜复合体中含有55种化合物,其中以烷烃、芳香烃、烯烃、酸类和醛类物质含量相对较高.另外,与正常植物相比,2-己烯醛、(+)-柠檬烯、α-石竹烯、DMNT和α-雪松烯等化合物仅在幼虫-植物复合体中产生或含量明显升高.[结论]淡足侧沟茧蜂更倾向于幼虫-玉米复合体,并且玉米和苋菜在遭受甜菜夜蛾取食后,会释放出更多种类的化合物.
Attractiveness of two host plants to Microplitis pallidipes and their volatile compounds identification
[Aim]To better understand the effect of host plants and Spodoptera exigua on the tendency behavior of Microplitis pallidipes.[Methods]Maize and amaranth were chosen and the behavioral responses of M.pallidipes to normal plants and larva-plant complex were determined with Y-tube olfactometer,and the volatile compounds were analyzed and identified by GC-MS.[Results]The normal maize and amaranth had no attraction effect on M.pallidipes,but larva-maize complex and larva-amaranth complex exhibited significant attractiveness,and larva-maize complex showed stronger activity than larva-amaranth complex.GC-MS indicated that 22 compounds were detected in normal maize leaves with alkanes and aromatic hydrocarbons as the main constitutes,while 31 compounds were identified in the larva-maize complex and mainly alkanes and alcohols.Similarly,there were 16 compounds in normal amaranth and the content of alkane compounds was the highest with 54.75%.The larva-amaranth complex contained 55 compounds,and alkanes,aromatics,alkenes,acids and aldehydes were relatively high.In addition,some compounds were only produced or increased remarkably in larva-plant complex,such as 2-hexenal,(+)-limonene,α-caryophyllene,DMNT,and α-himachalene.[Conclusion]M.pallidipes were prefer the larva-maize complex,and a wider variety of compounds were released by maize and amaranth after feeding by Spodoptera exigua.

Microplitis pallidipesmaizeamaranthSpodoptera exiguaattractant effectGC-MS

张彤瑶、蒋杰贤、张浩、王金彦、尤春梅、陈义娟、季香云

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上海海洋大学水产与生命学院,上海 201306

上海市农业科学院生态环境保护研究所,上海 201403

上海润庄农业科技有限公司,上海 201403

淡足侧沟茧蜂 玉米 苋菜 甜菜夜蛾 引诱作用 GC-MS

上海市青年科技启明星计划上海市农科院卓越创新团队项目上海市青年科技英才"扬帆计划"项目

21QB1404100202201721YF1441100

2024

应用昆虫学报
中国昆虫学会 中国科学院动物研究所

应用昆虫学报

CSTPCD北大核心
影响因子:0.679
ISSN:0452-8255
年,卷(期):2024.61(1)
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