Screening of Tetranychus urticae populations for resistance to diafenthiuron and the mechanism of biochemical resistance to this pesticide
[Aim]To investigate the development of resistance to diafenthiuron and the mechanism responsible for this resistance,in Tetranychus urticae.[Methods]A susceptible population of T.urticae was continuously selected for resistance to diafenthiuron for 33 generations in a laboratory.The activity of carboxylesterases(CarEs),glutathione S-transferases(GSTs),Mixed-function oxidases(MFOs)and chitinases(chitinases)in this population was tested every six generations.[Results]The resistance index had reached 18.61 after 33 generations.The activity of CarEs,GSTs and MFOs in the resistant population were significantly higher(1.909,2.119 and 7.436-fold,respectively)than in the sensitive population(P＜0.05).However,there was no significant difference in the chitinase activity in the sensitive populations(0.594,P＜0.05).[Conclusion]Resistance of T urticae to diafenthiuron is mainly associated with MFOs and chitinase activity,although GSTs and CarEs also play a role.
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