Content Determination and Analysis of Imipenem,Meropenem,Vancomycin and Tei-coplanin in Human Serum Based on LC-MS/MS
Objective:To establish a method for simultaneous and rapid determination of imipenem,meropenem,vancomycin and teicoplanin in human serum based on liquid chromatography-tandem mass spectrometry(LC-MS/MS),and provide reference for the therapeutic drug monitoring in clinically infected patients.Methods:By using Agilent 1260 series liquid chromatograph and API 4000 triple tandem quadrupole mass spectrometer,Phenomenex KINETEX XB-C18(2.6 μm,3×50 mm)as the chromatographic column,0.1%formic acid-water and pure acetonitrile as the mobile phase,electrospray ionization(ESI),multiple reaction monitoring(MRM)combined with positive ion mode as the mass spectrometry conditions,a method for simultaneous and rapid determination of 4 antibacterial drugs(imipenem,meropenem,vancomycin and teicoplanin)in human serum was established.Results:The determination results showed that there were no interference peaks at the retention time of 4 antibacterial drugs and 2 internal standard substances;4 antibacterial drugs had good linear relationships within their respective concentration ranges,and their correlation coefficients(r2)were all greater than 0.99,and the S/N of the lowest limit of quantification were all greater than 10;the precision and accuracy test results showed that the inter-day and intra-day relative standard deviations(RSDs)of determination results of 4 antibacterial drugs were all less than 10.00%,and their relative errors(REs)were all within±10.00%;the recovery rates of determination results of 4 antibacterial drugs were all between 85.00%and 115.00%;the review of stability showed that the REs of determination results of 4 antibacterial drugs were all within±10.00%at different temperatures and storage times.Conclusion:LC-MS/MS can determine the contents of 4 antibacterial drugs(imipenem,meropenem,vancomycin and teicoplanin)in serum simultaneously and rapidly,and has high precision and accuracy and strong stability;therefore,it can provide better support for clinical therapeutic drug monitoring.
liquid chromatography-tandem mass spectrometrycontent determinationimipenemmeropenemvancomycinteicoplanin
万方数据
维普
