Objective:To establish the quality standard of Qinggan Mixture,and to provide reference for the quality control of manufacture of Qinggan Mixture.Methods:Three kinds of medicinal materials in Qinggan Mixture,namely,cortex moutan,radix scutellariae and fructus gardeniae,were identified qualitatively by thin-layer chromatography(TLC),and the content of baicalin in Qinggan Mixture was determined by high performance liquid chromatography(HPLC).Results:The TLC map of cortex moutan,radix scutellariae and fructus gardeniae was clear in spots,with good separation effect,and strong specificity,and there was no interference from the negative control.When the mass concentration of baicalin was 16.125-258 μg/mL,it exhibited a good linear relationship with the peak response value(Apeak responsevalue=22655 ρμg/mL-99737,r2=0.9994,n=5).The average spiked recovery rate was 100.55%(RSD=1.92%);the average content of baicalin in three batches of Qinggan Mixture was 1.20 μg/mL.Conclusion:Cortex moutan,radix scutellariae and fructus gardeniae in the Qinggan Mixture can be qualitatively identified by TLC;HPLC has good specificity,precision,repeatability and stability in quantitative determination of baicalin in the Qinggan Mixture,and can be used for quantitative quality control of baicalin in the Qinggan Mixture.
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