Research on Anti-inflammatory Effects of the Fresh Oldenlandia Diffusa Water Extract and Alcohol Extract
Objective:To compare the differences in the anti-inflammatory effects in vivo and in vitro of fresh Hedyotis diffusa water extract and alcohol extract,so as to lay a theoretical foundation for further clinical studies.Methods:Fresh Hedyotis diffusa was subjected to refluxing extraction with water or 75%ethanol,and the extract was concentrated and freeze-dried to obtain the extract powder.The extract powder was prepared into the corresponding herbal medicine solution,then the anti-inflammatory effects on mouse macrophages(RAW264.7)and mice in an inflammatory model were observed,and the differences between the water extract and alcohol extract were compared.Results:Both the water extract and the alcohol extract of fresh Hedyotis diffusa inhibited the viability of lipopolysaccharide-induced RAW264.7,and the water extract was generally superior to the alcohol extract in terms of the inhibition rate of inflammatory factors such as interleukin-6,interleukin-1β,and tumor necrosis factor-α.In addition,both the water extract and the alcohol extract of fresh Hedyotis diffusa reduced ear swelling and writhing times in inflammatory model mice.The overall effect of the water extract was superior to the alcohol extract.Conclusion:The anti-inflammatory effects of the water extract and the alcohol extract of Hedyotis diffusa are different,and the former was superior to the latter,which may be correlated with the difference in their components.
万方数据
维普
