Study on Determination of Plasma Concentration of Ceftazidime-Avibactam Sodium by LC-MS/MS
Objective:To establish a method for determination of plasma concentration of ceftazidime-avibactam sodium by liquid chromatography-tandem mass spectrometry(LC-MS/MS),and provide a reference for the safe and rational use of ceftazidime-avibactam sodium in clinical practice.Methods:A method for determination of plasma concentration of ceftazidime-avibactam sodium by LC-MS/MS was established by using an ACQUITY UPLC BEH C18 column(2.1 mm×50 mm,1.7 μm),10 mmol/L ammonium acetate(A)-100%acetonitrile(B)as a mobile phase,a flow rate of 0.3 mL/min,and a positive/negative ionization switching multiple reaction monitoring(MRM)mode,etc.,and the specificity,precision,accuracy,recovery,stability of the method were tested.Results:The retention times of ceftazidime and avibactam were 0.89 min and 0.46 min,respectively.Endogenous substances in plasma had no interference with the detection of ceftazidime and avibactam.The linear equation of ceftazidime was y=537.989x+56.449 6(r=0.995 3),with good linear performance within the concentration range of 0.5-200μg/mL;the linear equation of avibactam was y=1 788.74x+25.226 9(r=0.998 0),with good linear performance within the concentration range of 0.05-25μg/mL.The intra-batch and inter-batch precisions of high-,medium-and low-concentration ceftazidime and avibactam samples were all within 15.00%,and their accuracy was within±15.00%.The recoveries of ceftazidime and avibactam in this method were above 80.00%.In addition,when the samples were stored at room temperature for 6 h,stored in the sample injection tray for 24 h,stored at 4℃for 24 h and repeatedly frozen and thawed(-80℃→25℃)for 3 cycles,the measurement deviations of high-,medium-and low-concentration ceftazidime and avibactam samples were all within±15.00%.Conclusion:This method has good specificity and a large linear range,and has good precision,accuracy and stability,which can provide a basis for the individualized medication of ceftazidime-avibactam sodium in clinical patients.
ceftazidime-avibactam sodiumplasma concentrationliquid chromatography-tandem mass spectrometry(LC-MS/MS)
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