Objective:To optimize the extraction process of Qinggan Mixture through orthogonal experimental design,and provide a reference for the production and quality control of Qinggan Mixture.Methods:The orthogonal experimental design was used to investigate the frequency of decoctions,decoction time and water addition in the extraction process of Qinggan Mixture;the dry extract yield,baicalin transfer rate and comprehensive cost of process were used as evaluation indicators to select and validate the optimal process route.Results:The orthogonal experimental design showed that the optimal route for the extraction process of Qinggan Mixture was as follows:3 times of decoctions,1 hour of decoction time,and 8 times of water addition.Under the optimal route,the dry extract yield(33.56%),baicalin transfer rate(80.05%)and comprehensive cost of process were ideal,and the process stability and repeatability were good.Conclusion:The extraction process route of Qinggan Mixture selected by the orthogonal experimental design has good stability and reproducibility,and can be used for the production of Qinggan Mixture.
关键词
清肝合剂/提取工艺/正交试验设计/黄芩苷转移率/干浸膏得率
Key words
Qinggan Mixture/extraction process/orthogonal experimental design/baicalin transfer rate/dry extract yield
维普
万方数据