Enrichment of benzopyrene anaerobic degrading bacteria and optimization of its conditions
The YQ-BaP microbial community with Benzo[a]pyrene(BaP)as the sole carbon source was enriched from the sludge straw combined anaerobic sludge system.The effects of different environmental factors on the biodegradability of bacteria were studied by single factor experiment.The intermediate metabolites degraded by benzo[a]pyrene were analyzed by gas chromatography-mass spectrometry(GC-MS).The results of macrogene sequencing showed that the dominant genera of this bacterium were Pseudomonas and Methyloversatilis.After 14 days of culture,the degradation rate of 30 mg/L BaP was stable at 38.69％±6.24％,and the degradation effect of phenanthrene and pyrene was better.YQ-BaP showed strong degradation ability at pH=7 and 35～40℃.When the concentration of BaP was 20 mg/L,the degradation rate of BaP reached 46.89％.GC-MS analysis showed that phenanthrene,anthracene,naphthalene and other hydroxylation and methylation metabolites were produced during BaP degradation.The bacterial community has a strong ability to degrade BaP,which provides a theoretical basis for the remediation technology of PAHs pollution.
benzo[a]pyreneanaerobic sludgeanaerobic degradation consortiumdegradation productsmicrobial community structure
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