Based on the characteristics of PCR technology,the game of simulating the PCR reaction process for students to performing was designed after simulated DNA molecules and simulated primers being designed.Encourage students to participate in a game that simulates the PCR reaction process,design routine questions and expand their thinking depth based on the game context,guide students to actively participate in classroom teaching activities through collaborative exploration,use both hands and brains,deeply understand the principles of PCR technology on the basis of critical thinking,and enhance students'awareness of using interdisciplinary knowledge to solve problems.