Innovative Method of Manufacturing Uniform Cell Spheroids Based on Thermal Inkjet Technology
Cell spheroids play the important role in drug discovery,physiological and pathological research,tissue en-gineering and other fields.However,the traditional method of manufacturing cell spheroids limits its application.Here,we propose a systematic method based on thermal inkjet printing technology,called Assembling Printing,and com-plete the construction of the printing system,which can quickly,high-throughput and low-cost produce uniform size spheroids.By using the Assembling Printing,864 cell spheroids with uniform diameter can be produced in 16 minutes.We took CHO cells as the research object and carefully analyzed the cell activity after printing.At the same time,in order to improve the uniformity of cell spheroids size,we proposed three optimization methods,which can reduce the coefficient of variation of cell spheroids diameter by 17.04%-48.38%.Finally,we studied the growth of printed cell spheroids cultured for 0,1,4,7 days.The experimental results fully demonstrate the advantages of the Assembling Printing system in the manufacturing of cell spheroids,and this method also provides reference significance for the sys-tematic study of organoids.
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