酿酒酵母色醇生物合成途径及分子调控机制研究进展
Research Progress on the Biosynthesis Pathways and Molecular Regulatory Mechanism of Tryptophol in Saccharomyces Cerevisiae
吴恒 1赵伟 2赵杨 2肖永银 1杨柳 2秦云华 2杜超 1罗义勇 3巩效伟2
作者信息
- 1. 云南中烟新材料科技有限公司,云南 昆明 650106
- 2. 云南中烟工业有限责任公司 技术中心,云南 昆明 650231
- 3. 江西师范大学 生命科学学院,江西 南昌 330022
- 折叠
摘要
色醇(Tryptophol,TOL),是一种具有多种生物活性的吲哚衍生物,也是合成二甲基色胺、吲哚拉明等药物的重要原料,在医药、食品、饲料和种植等领域均有巨大的应用价值.目前,色醇主要通过化学合成获取,但化学合成难以提纯,且容易造成环境污染.因此,绿色环保的生物合成法成为研究的热点.本文通过对产色醇微生物、酿酒酵母色醇从头合成途径和艾利希途径及其分子调控机制进行综述,并对现有研究的不足和今后研究方向进行探讨,以期为构建高产色醇工程菌株提供参考,也为通过代谢工程改造酿酒酵母以实现天然色醇的工业化生产成为可能.
Abstract
Tryptophol(TOL),an indole derivative with diverse biological activities,serves as a crucial precursor for the synthesis of drugs such as dimethyltryptamine and indoramin.It holds significant application value in vari-ous fields,including medicine,food,feed,and agriculture.Currently,TOL is primarily obtained through chemical synthesis,which,however,faces challenges in purification and may lead to environmental pollution.Therefore,en-vironmentally friendly biosynthesis has become a research hotspot.This paper provides a comprehensive review of microorganisms producing TOL,de novo biosynthetic pathways in Saccharomyces cerevisiae for TOL,the Ehrlich pathway,and their molecular regulatory mechanisms.The shortcomings of existing research are discussed,and po-tential future research directions are explored.This study aims to serve as a reference for the construction of high-yield TOL engineering strains and to pave the way for industrial-scale production of natural TOL through met-abolic engineering of Saccharomyces cerevisiae.
关键词
色醇/酿酒酵母/生物合成途径/调控机制Key words
tryptophol/Saccharomyces cerevisiae/biosynthesis pathway/regulatory mechanism引用本文复制引用
基金项目
国家自然科学基金(32060531)
云南中烟工业有限责任公司科技项目(2022XY01)
云南省科技项目(202101AT070279)
出版年
2024
NETL
NSTL
维普
万方数据