Allelopathic Effect of Phenolic Acid Autotoxins from Panax Notoginseng Rhizosphere on Two Pathogenic Fungi of Root Rot
The effects of four phenolic acids(benzoic acid,ferulic acid,syringic acid,p-coumaric acid)and their mixed phenolic acids on mycelial growth and spore germination of Fusarium solani and Fusarium equiseti were studied to further explore the interaction between phenolic acids and pathogens in rhizosphere soil of Panax Notog-inseng.The mycelial growth test showed that high concentration of ferulic acid significantly promoted the mycelial growth of F.solani after 6 days of treatment,and benzoic acid had a concentration effect of low promotion and high inhibition on F.solani after 6 days of treatment.At the same time,1.2-3.6 μg/mL syringic acid significantly pro-moted the mycelial growth of F.equiseti at 3d and 9d,and high concentration of benzoic acid showed a time effect of initial inhibition and later promotion on F.equiseti.The results of concentration for 50%of maximal effect(EC50)calculation showed that ferulic acid had the strongest promoting effect on F.solani and F.equiseti.The spore germi-nation test showed that p-coumaric acid,0.06-3.6 μg/mL syringic acid and benzoic acid at the later stage of culture all promoted the spore germination of F.solani.The four phenolic acids also showed a significant promoting effect on F.equiseti.The mixed phenolic acids had different allelopathic effects on the two pathogens,and showed a concentration effect.The high concentration of mixed phenolic acids significantly inhibited the mycelial growth of F.solani at 9 days of culture,but promoted the mycelial growth of F.equiseti.At the same time,the mixed phenolic acids promoted the spore germination of F.solani,but the promotion effect on F.equiseti was more significant at low concentrations.In summary,the four phenolic acids showed allelopathic promotion effects on the spore germination of F.solani and F.equiseti,but the interaction of different phenolic acids and their mixed phenolic acids on the two pathogens of P.notoginseng root rot showed a dynamic effect with concentration and time.
rhizosphere of Panax Notoginsengphenolic acidallelopathic effectcontinuous cropping obstacleroot rot
NETL
NSTL
万方数据
维普
