LC-MS/MS-Based Metabolomics of Electro-Thumbtack Needle in High-Altitude Exposed Population
With the development of western China and the tourism industry,the number of people traveling to high-altitude areas has been increasing,and the pathological changes caused by high-altitude exposure have a serious impact on human health.Previous clinical experience has shown that electro-thumbtack needle is effec-tive in intervening the symptoms of high-altitude exposure,but the mechanism of its action is still unclear.In this study,we investigated the metabolic mechanism of electro-thumbtack needle in regulating the symptoms of high-altitude exposure based on untargeted metabolomics.The recruited high-altitude exposed population was randomized into electro-thumbtack needles and sham acupuncture groups.The electro-thumbtack needles(ETN)group was intervened with electro-thumbtack needles,while the sham acupuncture(SA)group was in-tervened with blunt-tipped sham electro-thumbtack needles.10 people in the ETN group and 16 people in the SA group were finally included.The protocol will involve routine disinfection of the skin and stimulation of 7 acu-puncture points Bilateral Nei Guan(PC6),Bilateral He Gu(LI4),Bilateral Tai Yang(EX-HN5),and Zhong Wan(RN12)with pressure for approximately 1 minute each,and the intervention was continued for 48 hours.Evaluate the 2018 Lake Louise score,blood pressure,peripheral oxygen saturation,heart rate and other related indicators after intervention.The salivary difference metabolites before and after the trial intervention and between groups were tested and further pathway analysis was performed.Compared with the SA,the ETN showed a more significant decrease in the Lake Louise score after the intervention(P＜0.05).After screening for dif-ferential metabolites using untargeted metabolomics and conducting pathway enrichment analysis,three signifi-cantly enriched metabolic pathways were discovered,including D-amino acid metabolism,porphyrin metabo-lism,and glycine,serine,and threonine metabolism.There are four significantly different metabolites in three metabolic pathways:biliverdin,5-aminolevulinic acid,D-ornithine,and D-serine.The discovery of these pathways provides important clues for further research on their mechanisms of action and also offers new ideas for developing treatment methods for high-altitude diseases.
acupunctureelectro-thumbtack needleacute-mountain sicknessmetabolomicshigh altitude exposure people
万方数据
维普
