Detection of Physiological Indicators of Panax vietnamensis Ha &Grushv.in Response to Neofusicoccum ribis Infection and Identification of the Physiological Characteristics of N.ribis
Panax vietnamensis Ha&Grushv.is a medicinal plant with high added value in Yunnan Province,which is easily affected by diseases in the process of cultivation.Leaf blight is one of the most dangerous disease of P.vietnamensis,and its pathogen has been identified as Neofusicoccum ribis(N.ribis).In this study,the patho-genicity of N.ribis was evaluated by fungal inoculation experiment.Two days after inoculation,the symptoms of P.vietnamensis began to occur,which confirmed that N.ribis had strong infection ability to P.vietnamensis.The physiological indexes of P.vietnamensis after suffering from leaf blight were analyzed,the results showed that chlo-rophy gradually decreased with the increase of the N.ribis infection time,the contents of peroxidase,superoxide dismutase,catalase and reactive oxygen species in P.vietnamensis increased sharply in four days after inoculation and reached the peak on the fourth day.In addition,the biological characteristics of N.ribis were identified and analyzed,and it was found that the optimum growth medium was PDA,the optimum carbon source was fructose,the optimum nitrogen source was sodium nitrate,the optimum temperature was 25～30 ℃,the light was beneficial to its growth,and the optimum pH was 4.0～7.0.The results will lay a foundation and provide research ideas for the prevention and control of leaf blight in P.vietnamensis.
NETL
NSTL
万方数据
