目的:检测核基质蛋白特异AT序列结合蛋白2(SATB2)在口腔鳞癌组织中的表达,探讨其对口腔鳞癌细胞增殖的影响.方法:采用实时定量RT-PCR和Western blot分别检测口腔鳞癌组织及HN4细胞系中SATB2的基因和蛋白的表达情况;采用免疫荧光染色检测SATB2在HN4细胞系中的分布情况;通过转染慢病毒的方法过表达SATB2后,CCK-8实验检测其对口腔鳞癌细胞增殖能力的影响,流式细胞术检测细胞周期,Western blot 检测细胞周期相关蛋白P63、Cyclin B1及细胞内STAT3磷酸化水平;构建裸鼠荷瘤实验模型,观察其对移植瘤生长的影响.结果:SATB2在口腔鳞癌组织及HN4细胞系中呈高表达,而在癌旁组织和人口腔角质细胞中呈低表达(P<0.05);SATB2基因过表达后显著促进口腔鳞癌细胞的增殖能力,上调细胞周期相关蛋白P63、Cyclin B1,细胞内STAT3磷酸化明显上升,促进体内移植瘤的生长(P<0.05).结论:SATB2在口腔鳞癌组织及细胞系中表达升高,过表达SATB2能促进肿瘤细胞的增殖能力.
Expression of SATB2 in oral squamous cell carcinoma and its role in promoting cell poliferation
Objective: To investigate expression of special AT-rich sequence-binding protein 2(SATB2) in oral squamous cell carcinoma (OSCC)tissue samples,and then to study the proliferation roles of SATB2 in OSCC cells.Methods: The expression of SATB2 in OSCC and HN4 cell lines are tested by Western blot and qRT-PCR,cellular immunofluorescence was performed to further characterize the subcellular distribution of SATB2 in HN4 cell lines.Using lentivirus overexpressed SATB2,in vitro,cell proliferation were assessed by CCK-8 experiments and the cell cycles were measured by flow cytometry,the protein change of cell cycle-related protein P63,Cyclin B1 and STAT3 phosphorylation were tasted by Western blot.In vivo,the growth of tumor transplation with HN4 cells and Lv-SATB2-HN4 cells in nude mice was further observed.Results: The expression of SATB2 in OSCC and HN4 cell lines are tested by Western blot and qRT-PCR,and expression of SATB2 in OSCC tissues is higher than para-carcinoma tissues and expression of SATB2 in HN4 cells is higher than the oral keratin forms cells (P<0.05).CCK-8 assay,flow cytometry and xenograft model showed that overexpression SATB2 in HN4 cell lines significantly promoted cell proliferation,cell cycle-related protein P63,Cyclin B1 had been significantly up-regulated and STAT3 phosphorylation obviously enhanced.Conclusions: SATB2 was highly expressed both in OSCC tissues and HN4 cell lines.Either in vitro or in vivo,overexpression of SATB2 promoted cell proliferation.
NETL
NSTL
万方数据
维普
