首页|根尖牙乳头干细胞来源凋亡囊泡对巨噬细胞糖酵解相关酶的表达及炎症表型影响的初探研究

根尖牙乳头干细胞来源凋亡囊泡对巨噬细胞糖酵解相关酶的表达及炎症表型影响的初探研究

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目的:探究根尖牙乳头干细胞(SCAP)来源的凋亡囊泡(apoVs)是否通过影响糖酵解相关酶调控巨噬细胞(BMDMs)炎症表型,进而对炎症反应产生调节作用.方法:体外培养鉴定人源SCAP,经星形孢菌素诱导凋亡后提取apoVs,利用扫描电镜、流式细胞术等对其进行表征鉴定.分别空白处理、脂多糖(LPS)处理、LPS同apoVs共处理大鼠骨髓来源的BMDMs,利用流式细胞术检测促炎表型BMDMs表面标志分子CD80、CD86 的表达,利用Western blot检测BMDMs糖酵解相关酶的表达.结果:根尖牙乳头干细胞来源的apoVs能被BMDMs摄取,促炎表型标志分子CD80、CD86 的表达下调,糖酵解相关酶葡萄糖转运蛋白(GLUT)1、GLUT3 的表达显著下调(P<0.05).结论:SCAP来源apoVs能降低促炎型BMDMs的分布,并对其糖酵解相关酶的表达产生影响.
ApoVs derived from SCAP modulate the inflammatory phenotype of macrophages by regulating the expression of glycolysis-associated enzymes
Objective:To investigating whether apoptotic vesicles(apoVs)derived from stem cell from apical papilla(SCAP)modulate macrophage inflammatory phenotype by influencing glycolysis-associated enzymes,thereby regulating the inflammatory re-sponse.Methods:Human-derived SCAP were cultured and characterized in vitro.Staurosporine induction was employed to obtain apoVs from SCAP,followed by the characterization and identification of the apoVs using scanning electron microscopy,flow cytometry,and other methods.Experimental groups included a blank control group,an inflammation group and an apoVs group.Rat bone marrow-derived macrophages(BMDMs)were treated with PBS,LPS and LPS in conjunction with apoVs.Using flow cytometry to assess the ex-pression of surface markers CD80 and CD86 on pro-inflammatory macrophages;employing Western blot to detect the expression of gly-colytic enzymes in macrophages.Results:The apoVs derived from SCAP can be taken up by macrophages,reducing the expression of pro-inflammatory phenotype markers CD80 and CD86.Additionally,they exert an impact on glycolysis-associated enzymes,with a sig-nificant downregulation observed in the expression of GLUT1 and GLUT3(P<0.05).Conclusions:The apoVs derived from SCAP reg-ulate the expression of glycolysis-associated enzymes in macrophages,reducing the distribution of pro-inflammatory phenotype macro-phages,thereby modulating the inflammatory response.

stem cells from apical papillamacrophagesapoptotic vesiclesglycolysis

曾涵柔、李长芳、王可境、张婧、骞爱荣、胡丽芳、王玮

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西北工业大学生命学院,陕西 西安 710072

第四军医大学口腔医院牙体牙髓病科,口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔医学重点实验室,陕西 西安 710032

第四军医大学口腔医院口腔解剖生理学教研室,陕西 西安 710032

根尖牙乳头干细胞 巨噬细胞 凋亡囊泡 糖酵解

国家自然科学基金面上项目陕西省重点研发计划项目陕西省重点研发计划项目

823709422021SF-0322022SF-441

2024

口腔生物医学
南京医科大学

口腔生物医学

CSTPCD
影响因子:0.423
ISSN:1674-8603
年,卷(期):2024.15(1)
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