Molecular mechanism of ANGPTL4 promoting the metastasis of tongue cancer cells by regulating epithelial-mesenchymal transition
Objective:To explore the molecular mechanism of ANGPTL4 in the progression and lymph node metastasis of tongue cancer,in order to provide a new molecular target for the treatment of tongue cancer.Methods:The ANGPTL4 gene in tongue cancer cells was silenced by small interfering RNA(siRNA).The transfection efficiency was tested by qRT-PCR.The biological behavior of tongue cancer cells after ANGPTL4 silencing was determined by CCK-8,Transwell and scratch experiments.The expressions of epithe-lial-to-mesenchymal transition(EMT)related markers were detected by reverse transcription polymerase chain reaction and Western blotting.Results:After silencing ANGPTL4 by siRNA,the proliferation was significantly reduced(P＜0.01)and migration ability of tongue cancer cells was weakened(P＜0.05).The expression of downstream related genes of EMT,E-cadherin was up-regulated and Vimentin,DDR1,ZEB-1 were down-regulated.Conclusions:ANGPTL4 can enhance the adhesion between tongue cancer cells,there-by reducing the migration and epithelial-mesenchymal transition of tongue cancer cells.ANGPTL4 may be a potential target for lymph node metastasis of tongue cancer.
angiopoietin like protein 4tongue cancerproliferationmigrationlymphatic metastasis
万方数据
维普
