首页|Has_circ_0008717在羊膜间充质干细胞上清促血管生成中的作用研究

Has_circ_0008717在羊膜间充质干细胞上清促血管生成中的作用研究

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目的:探究has_circ_0008717 在人羊膜间充质干细胞上清(hAMSC-CM)促进人脐静脉内皮细胞(HUVECs)血管生成过程中的作用.方法:通过划痕实验、Transwell实验及成管实验检测hAMSC-CM对HUVECs迁移及成管能力的影响,实时荧光定量RT-PCR筛选上调的circRNA(has_circ_0008717);小干扰RNA(siRNA)下调HUVECs内has_circ_0008717 水平后,实时荧光定量RT-PCR检测血管内皮生长因子A(VEGFA)表达水平变化,划痕实验、Transwell实验及成管实验观察其对hAMSC-CM促进HUVECs迁移及成管的影响.结果:hAMSC-CM可明显促进HUVECs迁移及成管能力(P＜0.05),HUVECs中has_circ_0008717 升高最为明显(P＜0.05),而敲低has_circ_0008717 可明显抑制hAMSC-CM促进HUVECs迁移、成管和VEGFA的表达(P＜0.05).结论:hAMSC-CM可通过has_circ_0008717 增强HUVECs的成管能力.
The function of has_circ_0008717 in pro-angiogenic capacity of conditioned medium from human amnion-derived mesenchy-mal stem cells
Objective:To investigate the role of has_circ_0008717 during the process that human amnion-derived mesenchymal stem cells(hAMSCs)promote angiogenesis of human umbilical vein endothelial cells(HUVECs).Methods:Wound healing assay,Transwell migration and tube formation were used to confirm the pro-angiogenic capacity of conditioned medium from hAMSCs(hAMSC-CM)on HUVECs,qRT-PCR and were used to detect the change of level of the potential pro-angiogenic relative circRNAs and genes in HUVECs after treated with hAMSC-CM,among them,has_circ_0008717 and vascular endothelial growth factor A(VEG-FA)attracted our attention.siRNAs specially targeted has_circ_0008717 were used to down-regulate the level of has_circ_0008717 in HUVECs treated with hAMSC-CM,and then qRT-PCR was used to detect were used to evaluate the change of level of VEGFA caused by down-regulation of has_circ_00087,afterwards,wound healing assay,Transwell migration and tube formation were used to assess the effects of down-regulation of has_circ_0008717 on HUVECs treated with hAMSC-CM.Results:Enhanced migration and tube formation in HUVECs induced by hAMSC-CM were observed(P＜0.05).And has_circ_0008717 been the mostly up-regulated circRNA(P＜0.05).Increased migration,tube formation and expression of VEGFA was observed(P＜0.05).Furthermore,these can be inhibi-ted by silencing has_circ_0008717 in HUVEC treated with hAMSC-CM(P＜0.05).Conclusion:has_circ_0008717 might be involved in the pro-angiogenic role of hAMSC-CM in HUVECs.

has_circ_0008717amnion-derived mesenchymal stem cellsangiogenesisvascular endothelial growth factor A

翟家彬、袁乙钦、高洋、陆史俊、钱文慧、唐子春

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南京大学附属口腔医院·南京市口腔医院急诊综合科,南京大学口腔医学研究所,江苏 南京 210029

苏州大学苏州医学院,江苏 苏州 215005

苏州大学附属第一医院口腔科,江苏 苏州 215005

苏州市立医院口腔科,江苏 苏州 215005

苏州口腔医院口腔颌面外科,江苏 苏州 215005

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has_circ_0008717 羊膜间充质干细胞 血管生成 血管内皮生长因子A

苏州市科技发展项目苏州市科技发展项目苏州市卫生人才项目江苏省口腔疾病研究重点实验室开放基金

SKJY2021024SKYD2023077GSWS2022117JSKLOD-KF-2305

2024

10.3969/j.issn.1674-8603.2024.02.005

口腔生物医学
南京医科大学

口腔生物医学

CSTPCD
影响因子：0.423
ISSN：1674-8603
年,卷(期)：2024.15(2)
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