Has_circ_0008717在羊膜间充质干细胞上清促血管生成中的作用研究
The function of has_circ_0008717 in pro-angiogenic capacity of conditioned medium from human amnion-derived mesenchy-mal stem cells
翟家彬 1袁乙钦 2高洋 3陆史俊 4钱文慧 5唐子春5
作者信息
- 1. 南京大学附属口腔医院·南京市口腔医院急诊综合科,南京大学口腔医学研究所,江苏 南京 210029
- 2. 苏州大学苏州医学院,江苏 苏州 215005
- 3. 苏州大学附属第一医院口腔科,江苏 苏州 215005
- 4. 苏州市立医院口腔科,江苏 苏州 215005
- 5. 苏州口腔医院口腔颌面外科,江苏 苏州 215005
- 折叠
摘要
目的:探究has_circ_0008717 在人羊膜间充质干细胞上清(hAMSC-CM)促进人脐静脉内皮细胞(HUVECs)血管生成过程中的作用.方法:通过划痕实验、Transwell实验及成管实验检测hAMSC-CM对HUVECs迁移及成管能力的影响,实时荧光定量RT-PCR筛选上调的circRNA(has_circ_0008717);小干扰RNA(siRNA)下调HUVECs内has_circ_0008717 水平后,实时荧光定量RT-PCR检测血管内皮生长因子A(VEGFA)表达水平变化,划痕实验、Transwell实验及成管实验观察其对hAMSC-CM促进HUVECs迁移及成管的影响.结果:hAMSC-CM可明显促进HUVECs迁移及成管能力(P＜0.05),HUVECs中has_circ_0008717 升高最为明显(P＜0.05),而敲低has_circ_0008717 可明显抑制hAMSC-CM促进HUVECs迁移、成管和VEGFA的表达(P＜0.05).结论:hAMSC-CM可通过has_circ_0008717 增强HUVECs的成管能力.
Abstract
Objective:To investigate the role of has_circ_0008717 during the process that human amnion-derived mesenchymal stem cells(hAMSCs)promote angiogenesis of human umbilical vein endothelial cells(HUVECs).Methods:Wound healing assay,Transwell migration and tube formation were used to confirm the pro-angiogenic capacity of conditioned medium from hAMSCs(hAMSC-CM)on HUVECs,qRT-PCR and were used to detect the change of level of the potential pro-angiogenic relative circRNAs and genes in HUVECs after treated with hAMSC-CM,among them,has_circ_0008717 and vascular endothelial growth factor A(VEG-FA)attracted our attention.siRNAs specially targeted has_circ_0008717 were used to down-regulate the level of has_circ_0008717 in HUVECs treated with hAMSC-CM,and then qRT-PCR was used to detect were used to evaluate the change of level of VEGFA caused by down-regulation of has_circ_00087,afterwards,wound healing assay,Transwell migration and tube formation were used to assess the effects of down-regulation of has_circ_0008717 on HUVECs treated with hAMSC-CM.Results:Enhanced migration and tube formation in HUVECs induced by hAMSC-CM were observed(P＜0.05).And has_circ_0008717 been the mostly up-regulated circRNA(P＜0.05).Increased migration,tube formation and expression of VEGFA was observed(P＜0.05).Furthermore,these can be inhibi-ted by silencing has_circ_0008717 in HUVEC treated with hAMSC-CM(P＜0.05).Conclusion:has_circ_0008717 might be involved in the pro-angiogenic role of hAMSC-CM in HUVECs.
关键词
has_circ_0008717/羊膜间充质干细胞/血管生成/血管内皮生长因子AKey words
has_circ_0008717/amnion-derived mesenchymal stem cells/angiogenesis/vascular endothelial growth factor A引用本文复制引用
基金项目
苏州市科技发展项目(SKJY2021024)
苏州市科技发展项目(SKYD2023077)
苏州市卫生人才项目(GSWS2022117)
江苏省口腔疾病研究重点实验室开放基金(JSKLOD-KF-2305)
出版年
2024
NETL
NSTL
万方数据