Effect of constitutive activation of β-catenin on initial healing of extraction sockets
Objective:To investigate the effects of constitutive activation of β-catenin on the initial alveolar bone remodeling after tooth extraction.Methods:Catnblox(ex3)/+mice(control group)and 9.6 kb Dmp1-Cre+/-;Catnblox(ex3)/+mice(experimental group)were constructed.The right maxillary first molar was extracted and were collected 1 week later.Hematoxylin-eosin(HE)staining,Masson staining and tartrate resistant acid phosphatase(TRAP)staining were used to observe the healing of extraction sockets,and immunohis-tochemical staining was used to detect the differences in the expression of osteoblast-specific matrix proteins in the extraction sockets of the two groups of mice.Results:We successfully constructed 9.6kb Dmp1-Cre+/-;Catnblox(ex3)/+mice(experimental group).Compared with the control group,HE staining of the experimental groupshowed no neoplastic bone,Masson staining showed decreased collagen mineralization(P＜0.001),TRAP staining showed a decrease in the number of osteoclasts(P＜0.001),and immunohistochemical stai-ning showed reduced specific expression of osteocalcin(OCN),collagen typeⅠ(COL1),and dentin matrix protein(DMP1)in the cytoplasm of osteoblasts(P＜0.01).Conclusion:Constitutive activation of β-catenin in 9.6kb DMP1+cells delayed the initial healing rate of extraction sockets.
extraction socket healingalveolar bone remodelingβ-cateninWnt/β-catenin signaling pathway
万方数据
维普
