Effect of graphene oxide-chitosan-barium titanate/polyvinylidene fluoride trifluoroethylene piezoelectric composite coating on the osteogenic differentiation of human umbilical cord wharton's jelly-derived mesenchymal stem cell
Objective:To investigate the effect of graphene oxide,chitosan-modified barium titanate nanoparticles(BCG-NPs)and polyvinylidene fluoride trifluoroethylene(P(VDF-TrFE))composite piezoelectric materials on the osteogenic differentiation of hWJ-MSC.Methods:The BCG-NPs/P(VDF-TrFE)(BCGP-Ti)coating was prepared on the surface of titanium by drop coating method.The surface morphology,phase composition,hydrophilicity and piezoelectric properties of the coating were characterized.The ability of the samples to induce mineralization in vitro was tested by simulated body fluid immersion test.hWJ-MSC was inoculated on the surface of the sample,and the cell viability was detected by CCK-8.The adhesion of hWJ-MSC was observed through cytoskeleton staining.ALP and alizarin red staining were used to evaluate ALP activity and mineralization level.The expressions of osteogenic differentiation related genes and proteins were detected through qRT-PCR and immunofluorescence staining.The Ti rods and BCGP-Ti rods were im-planted into the femur of SD rats,respectively.Micro-CT and histological staining were applied to examine the osseointegration.Re-sults:The fabrication of the BCGP-Ti coating was confirmed by scanning electron microscope and X-ray diffraction analysis.After po-larization,BCGP-Ti showed good piezoelectric response and better hydrophilicity than that of Ti and unpolarized BCGP-Ti(P＜0.01),which induced better mineralization in vitro than those of the other two groups(P＜0.01).After polarization,the area of hWJ-MSC spreading on BCGP-Ti surface was higher than that on Ti surface(P＜0.05).Compared with Ti,polarized BCGP-Ti not only promoted the expression of osteogenic differentiation related genes including ALP,COLI,RUNX2,OCN and proteins(P＜0.05),but also achieved better osseointegration in vivo.Conclusions:BCG-NPs/P(VDF-TrFE)composite coating on the surface of Ti could promote osseointegration by inducing osteogenic differentiation of hWJ-MSC.
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