Phosphoprotein biomimetic analogue cross-linked collagen scaffold in vivo osteogenic efficacy
Objective:This study investigated the osteogenic efficacy of phosphorylated polyamideamine(P-PAMAM)dendritic macromolecules cross-linked to collagen scaffolds in SD rats.Methods:Fourier transform infrared spectroscopy(FTIR)was used to characterize the phosphorylation of PAMAM and the morphological identification of three groups of collagen scaffolds,namely collagen scaffold group,PAMAM composite scaffold group and P-PAMAM composite scaffold group.Micro-CT three-dimensional reconstruction was used to analyze the changes of bone mass during bone healing after skull defect implantation in rats with composite scaffolds.HE staining and Masson staining were used to analyze the skull specimens of rats.Results:FTIR showed that PAMAM was successfully phosphorylated;scanning electron microscopy showed that the composite scaffold pore formation was good;and Micro-CT analysis of bone volume,ratio of new bone mass to total defect volume,bone mineral density and trabecular bone count,HE staining and Masson staining showed that the P-PAMAM composite collagen scaffold had better osteogenic induction effect than the collagen scaffold and PAMAM composite scaffold.Conclusions:P-PAMAM dendritic macromolecule composite collagen scaffold has a good effect on osteo-genesis in vivo.
万方数据
