Study on the effect of salidroside on proliferation,migration and mineralization of mouse odontoblast cell line MDPC-23
Objective:To investigate the influence of salidroside(SAL)on the proliferation,migration and mineralization abilities of the mouse odontoblast cell line MDPC-23.Methods:MDPC-23 cells were cultured in vitro and stimulated with different concentra-tions of salidroside(0 μmol/L,10 μmol/L,20 μmol/L,50 μmol/L and 100 μmol/L).The effects of salidroside on MDPC-23 prolif-eration and migration were examined by using CCK-8,live-dead cell staining,scratch assay and Transwell assay.Three groups were set up:control group(osteogenic medium),and 10 μmol/L SAL group and 20 μmol/L SAL group.The mineralization capacity of SAL was evaluated by alkaline phosphatase(ALP)staining,ALP activity assay,alizarin red staining(ARS),calcium nodule quantifica-tion,qRT-PCR and western blot.Results:SAL promoted cell proliferation without significant cytotoxicity,with the most significant effect at 20 μmol/L(P＜0.05).SAL promoted cell proliferation across the experimental concentration range,with 20 μmol/L SAL ex-hibiting a significant proliferative effect.SAL had no significant cytotoxicity.SAL significantly enhanced cell migration(P＜0.05).SAL treatment resulted in a greater number of mineralized nodules(P＜0.05).ALP staining and activity assays indicated that 20 μmol/L SAL significantly promoted ALP expression(P＜0.05).The expression of ALP,osteocalcin(OCN),Runt-related transcription factor 2(Runx2),dentin sialophosphoprotein(DSPP),and dentin matrix protein-1(DMP-1)were significantly increased(P＜0.05),and the protein expression levels of OCN and Runx2 were also significantly increased after SAL treatment(P＜0.05).Conclusions:SAL can promote the proliferation,migration and mineralization abilities of odontoblast cell line MDPC-23 in mice.
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