A self-assembled layer on titanium surface via silane coupling of chlorogenic acid improves the osteogenic potential in a high-glucose environment
Objective:To explore the osteogenic potential and antioxidant ability of chlorogenic acid(CGA)coating on titanium surface in a high glucose environment.Methods:A bioactive coating was formed on the titanium surface by grafting CGA using AE-APTMS.The coating was characterized using scanning electron microscopy and a water contact angle instrument.Cell proliferation,ad-hesion to the coating,osteogenesis potential,reactive oxygen species(ROS)generation capability and the expression of osteogenesis-related genes was detected by real-time fluorescence quantitative PCR were evaluated in MC3T3-E1 cell line under high glucose condi-tions prior to ossification.Results:The successful grafting of chlorogenic acid onto the titanium surface resulted in a highly hydrophilic coating(P＜0.001).This coating effectively enhances cell proliferation and adhesion,while reducing ROS generation and promoting the expression of osteogenesis-related genes(P＜0.05).Conclusions:The chlorogenic acid coating on the titanium surface exhibits a poten-tial to mitigate ROS generation and enhance osteogenesis in a high glucose environment.
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维普
