Effects of bioactive glass on proliferation and YAP/TAZ expression of rat osteoblasts
Objective:To investigate the effects of bioactive glass(BG)on the proliferation of osteoblasts and the expression of YAP/PDZ-binding motif of transcriptional coactivator(TAZ).Methods:High density polyethylene(HDPE),hydroxyapatite(HA)+HDPE complex and BG+HDPE complex were constructed.The crystal morphology and structure of polymer phase in the three biomate-rials were observed by scanning electron microscopy(SEM)and wide-angle X-ray diffraction.The three materials were fixed in the bot-tom of the cell culture dish after ultraviolet disinfection,and the rat osteoblasts were inoculated and cultured in the dish for 7 days.The cell structure was observed by contrast microscope and laser confocal microscope,and the proliferation and apoptosis of the cells in each group were detected by CCK-8 and flow cytometry.Western blot was used to detect the expressions of alkaline phosphatase(ALP),collagen type Ⅰ(COL-1),osteopontin(OPN),Runt-associated transcription factor 2(Runx2),YAP and TAZ proteins in osteoblasts in each group.Results:The HDPE matrix phase of the three materials showed a typical orientation structure,and HA and BG were dispersed in the matrix phase.The constituent bone cells were long fusiform,the cell morphology was complete,the cytoskele-ton was continuous and complete without fracture or loss,and the cell nuclei were rounded and three-dimensional cells showed mitosis.The proliferation activity of bone cells composed of BG+HDPE was higher than that of the other two groups(P＜0.05),and the apopto-sis rate was lower than 5％in all three groups.The protein expressions of ALP,COL-1,OPN,Runx2,YAP and TAZ in bone cells composed of BG+HDPE were higher than those in the other two groups(P＜0.05).Conclusions:BG can promote the proliferation of osteoblasts in rats,and its mechanism may be related to the up-regulation of YAP/TAZ and Runx2 expression.
万方数据
维普
