Study on SEMA4D modified titanium surface modulating endothelial cell functions via macrophages
Objective To explore the direct impact of SEMA4D-modified titanium surface on macrophages and the indirect regulatory effect on endothelial cell functions through macrophages.Methods Pure titanium specimens were polished and cleaned,and SEMA4D coatings were prepared using alkali heat treatment and self-assembly techniques.The experimental group consisted of titanium specimens coated with SEMA4D solutions at concentrations of 25,50,and 100 ng/mL,with smooth titanium(Ti)specimens as the control group.The surface micromorphology and hydrophilicity of titanium in each group were analyzed by scanning electron microscopy and a contact angle meter.Macrophages were introduced onto the surface of each group,and the resulting supernatant was used to cul-ture endothelial cells.The proliferative activity of the cells was measured using CCK-8 assay.The inflammatory expression and VEGF expression of macrophages were evaluated using real-time quantitative PCR.The adhesion and migration abilities of endothelial cells were determined using the fluorescent staining and scratch assay.Results The SEMA4D-modified titanium surface showed a porous microstructure covered by an organic film,which was non-cytotoxic.This surface reduced macrophage inflammatory expression,up-reg-ulated VEGF expression and promoted endothelial cells'adhesion and migration.Conclusion SEMA4D modified titanium surface can reduce inflammatory response and enhance the motor function of endothelial cells,thereby obtaining a physiological microenvironment conducive to soft tissue repairing.
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