Effects of 10-MDP calcium salt on proliferation,apoptosis and MMPs expression of L929 fibroblasts
Objective To investigate the effects of different concentrations of 10-MDP calcium salts on biological behaviors of L929 fibroblasts in the periodontium.Methods The X-Ray diffraction(XRD)was used to examine 10-MDP calcium salts obtained by the three synthesized formulas.The cell proliferation and cytotoxicity of L929 was measured by CCK-8 method;the flow cytometry was used to detect cell apoptosis,and the protein expression of matrix metalloproteinase(MMP)-2 and MMP-9 was measured by Western blot.Results The XRD results confirmed that the 10-MDP calcium salts obtained by the three synthesized formulas were in different struc-tures.In the CCK-8 assay,only the 1.0 mg/mL MDP-1 group caused a decrease in cell proliferation activity after 72 h.No cell apopto-sis was induced in each group after 72 hours of culture.The secretion of MMP2 and MMP9 proteins in L929 cells were inhibited in 0.2 mg/mL and 0.1 mg/mL MDP-2 and MDP-1 groups.Conclusion 10-MDP calcium salts may not have a cytotoxic effect on the peri-odontium;they can exert an inhibitory effect on the destruction of the periodontium by inhibiting the secretion of MMP2 and MMP9.
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