10-MDP钙盐对小鼠成纤维细胞L929增殖、凋亡及MMPs表达的影响
Effects of 10-MDP calcium salt on proliferation,apoptosis and MMPs expression of L929 fibroblasts
周吕惠 1吴雨旻 2陈晨1
作者信息
- 1. 南京医科大学口腔疾病研究江苏省重点实验室,江苏南京(210029);南京医科大学附属口腔医院牙体牙髓科,江苏南京(210029)
- 2. 南京医科大学口腔疾病研究江苏省重点实验室,江苏南京(210029);南京医科大学附属口腔医院口腔修复科,江苏南京(210029)
- 折叠
摘要
目的 通过 10-MDP钙盐对小鼠成纤维细胞L929 的研究调查了不同浓度不同结构的 10-MDP钙盐对牙周组织的影响.方法 通过X射线衍射(X-Ray diffraction,XRD)对合成的三种不同比例 10-MDP 钙盐进行鉴定.使用 CCK-8 法检测10-MDP钙盐对小鼠成纤维细胞L929 的细胞增殖毒性;流式细胞术检测细胞凋亡;蛋白免疫印迹实验检测细胞基质金属蛋白酶(matrix metalloproteinase,MMP)2、MMP9 表达.结果 XRD的结果证实三种合成方式均有不同结构 10-MDP钙盐的产生;细胞增殖毒性CCK-8 实验中仅有 1.0 mg/mL MDP-1 组在 72h后引起细胞增殖活性降低;各组培养 72h后均不诱导细胞凋亡;0.2 mg/mL和 0.1 mg/mL MDP-2、MDP-1组均抑制L929 细胞的MMP2 和MMP9 蛋白分泌.结论 10-MDP钙盐可能对牙周组织中成纤维细胞没有细胞毒性作用,并且可以通过抑制MMP2、MMP9 蛋白的分泌减少牙周组织破坏.
Abstract
Objective To investigate the effects of different concentrations of 10-MDP calcium salts on biological behaviors of L929 fibroblasts in the periodontium.Methods The X-Ray diffraction(XRD)was used to examine 10-MDP calcium salts obtained by the three synthesized formulas.The cell proliferation and cytotoxicity of L929 was measured by CCK-8 method;the flow cytometry was used to detect cell apoptosis,and the protein expression of matrix metalloproteinase(MMP)-2 and MMP-9 was measured by Western blot.Results The XRD results confirmed that the 10-MDP calcium salts obtained by the three synthesized formulas were in different struc-tures.In the CCK-8 assay,only the 1.0 mg/mL MDP-1 group caused a decrease in cell proliferation activity after 72 h.No cell apopto-sis was induced in each group after 72 hours of culture.The secretion of MMP2 and MMP9 proteins in L929 cells were inhibited in 0.2 mg/mL and 0.1 mg/mL MDP-2 and MDP-1 groups.Conclusion 10-MDP calcium salts may not have a cytotoxic effect on the peri-odontium;they can exert an inhibitory effect on the destruction of the periodontium by inhibiting the secretion of MMP2 and MMP9.
关键词
10-MDP钙盐/牙周组织/基质金属蛋白酶/细胞凋亡Key words
10-MDP calcium salts/periodontium/matrix metalloproteinases/cell apoptosis引用本文复制引用
基金项目
国家自然科学基金(81970927)
江苏省重点研发计划社会发展项目(BE2022797)
江苏省科教能力提升工程——江苏省研究型医院建设单位(YJXYYJSDW4)
江苏省医学创新中心(CXZX202227)
出版年
2024
NETL
NSTL
维普
万方数据