首页|PTK7在口腔鳞癌中的表达分析及其生物学功能研究

PTK7在口腔鳞癌中的表达分析及其生物学功能研究

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目的 探讨PTK7 在口腔鳞癌(oral squamous cell carcinoma,OSCC)中的表达水平、潜在的生物学功能及其临床意义.方法 采用实时荧光PCR(quantitive real-time polymerase chain reaction,qPCR)和Western blot检测PTK7 在OSCC细胞系和口腔鳞癌组织标本中的表达情况,利用siRNA干扰技术下调PTK7 在HN6 和Cal27 细胞系中的表达,通过CCK-8实验、平板克隆实验、细胞划痕实验及Transwell实验检测PTK7 下调后其对OSCC细胞系增殖、迁移、侵袭的影响;同时采用免疫组织化学染色法检测 75 例口腔鳞癌组织中PTK7 蛋白的表达水平,分析其相关的临床意义.结果 qPCR及Western blot结果显示PTK7 基因及其编码蛋白在口腔鳞癌细胞系HN6 和Cal27 中高表达,并在 6 例新鲜口腔鳞癌标本中的表达高于其配对的癌旁正常组织;下调PTK7 的表达可抑制OSCC细胞增殖、迁移和侵袭功能;在 75 例口腔鳞癌组织中,PTK7 的表达水平与OSCC患者的发病年龄、吸烟情况、病理分化程度等相关,其差异有统计学意义(P<0.05).Kaplan-Meier生存分析发现,高表达PTK7的OSCC患者的预后较差.结论 PTK7 是口腔鳞癌发生发展过程的潜在癌基因,其表达水平影响OSCC细胞的生物学功能,临床上可根据PTK7 表达水平了解口腔鳞癌的特性,PTK7 可作为口腔鳞癌预后判定的独立指标.
Analysis of PTK7 expression in oral squamous cell carcinoma and its biological function
Objective To explore the expression level of PTK7 in OSCC,and study its potential biological function and clinical sig-nificance.Methods qPCR and Western blot were performed to detect the expression of PTK7 in OSCC cell lines and OSCC tissue specimens.siRNA interference technology was used to down-regulate the expression of PTK7 in HN6 and Cal27 cell lines.The effects of down-regulated PTK7 on the proliferation,migration,invasion of OSCC cells were detected by CCK-8 assay,plate cloning assay,cell scratch assay,Transwell assay.Immunohistochemistry was used to detect the expression level of PTK7 protein in 75 cases of OSCC tis-sue.Results The results of qPCR and Western blot showed that PTK7 gene and its coding protein were highly expressed in OSCC lines HN6 and Cal27,and the expression of PTK7 gene and its coding protein was higher in 6 fresh OSCC samples than in their paired adjacent normal tissues.Down-regulating the expression of PTK7 could inhibit the proliferation,migration and invasion of OSCC cells.In the 75 OSCC specimens,the expression level of PTK7 was significantly associated with the patients'age,smoking and differentiation degree(P<0.05).Kaplan Meier survival analysis found that OSCC patients with high expression of PTK7 had poorer prognosis.Con-clusion PTK7 is a potential oncogene in the occurrence and development of OSCC,and its expression level affects the biological func-tion of OSCC cells.The characteristics of OSCC can be understood clinically according to the expression level of PTK7,and PTK7 can be used as an independent prognostic indicator for OSCC.

oral squamous cell carcinomaprotein tyrosine kinase 7proliferationmigrationinvasion

孙昕奕、潘玥彤、陆欣悦、吕中静、袁健、李家锋、石欢

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徐州医科大学口腔医学院,江苏徐州(221004)

徐州医科大学附属医院口腔科,江苏徐州(221006)

上海交通大学医学院附属第九人民医院口腔外科,上海(200125)

口腔鳞癌 蛋白酪氨酸激酶7 增殖 迁移 侵袭

江苏省高等学校大学生创新创业训练计划徐州医科大学附属医院院级课题

202210313102Y2020KB008

2024

口腔医学
南京医科大学口腔医学院

口腔医学

CSTPCD
影响因子:0.641
ISSN:1003-9872
年,卷(期):2024.44(4)
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