Analysis of PTK7 expression in oral squamous cell carcinoma and its biological function
Objective To explore the expression level of PTK7 in OSCC,and study its potential biological function and clinical sig-nificance.Methods qPCR and Western blot were performed to detect the expression of PTK7 in OSCC cell lines and OSCC tissue specimens.siRNA interference technology was used to down-regulate the expression of PTK7 in HN6 and Cal27 cell lines.The effects of down-regulated PTK7 on the proliferation,migration,invasion of OSCC cells were detected by CCK-8 assay,plate cloning assay,cell scratch assay,Transwell assay.Immunohistochemistry was used to detect the expression level of PTK7 protein in 75 cases of OSCC tis-sue.Results The results of qPCR and Western blot showed that PTK7 gene and its coding protein were highly expressed in OSCC lines HN6 and Cal27,and the expression of PTK7 gene and its coding protein was higher in 6 fresh OSCC samples than in their paired adjacent normal tissues.Down-regulating the expression of PTK7 could inhibit the proliferation,migration and invasion of OSCC cells.In the 75 OSCC specimens,the expression level of PTK7 was significantly associated with the patients'age,smoking and differentiation degree(P<0.05).Kaplan Meier survival analysis found that OSCC patients with high expression of PTK7 had poorer prognosis.Con-clusion PTK7 is a potential oncogene in the occurrence and development of OSCC,and its expression level affects the biological func-tion of OSCC cells.The characteristics of OSCC can be understood clinically according to the expression level of PTK7,and PTK7 can be used as an independent prognostic indicator for OSCC.