摘要
目的 探讨医用臭氧注射疗程对SD大鼠颞下颌关节骨关节炎及其疼痛作用的影响.方法 按随机数字表法将 54只大鼠分为对照组、模型组、医用臭氧组,每组 18 只.对照组在造模时仅注射生理盐水;模型组仅注射碘乙酸钠造模;医用臭氧组关节上腔注射碘乙酸钠造模 1 周后关节内注射医用臭氧进行干预,注射频率为 1 次/周,共 5 次.注射医用臭氧 1 周(造模后 2 周)、3 周(造模后 4 周)、5 周(造模后 6 周)后,各组分别处死 6 只大鼠.处死大鼠前检测各组大鼠机械缩头阈值,处死后检测各组大鼠关节液中白介素-1β(interleukin-1β,IL-1β)表达水平,并对各组大鼠颞下颌关节软骨大体观进行Pelletier评分和软骨苏木精-伊红染色后进行改良Mankin's评分.结果 同一时间内:模型组相比于对照组,1、3、5 周,大鼠颞下颌关节骨关节机械痛阈值减小(P＜0.01),大鼠颞下颌关节液中IL-1β表达水平升高(P＜0.01),颞下颌关节软骨Pelletier 评分以及软骨改良Mankin's评分增高(P＜0.01);医用臭氧组相比于模型组,注射医用臭氧 3 周和 5 周,大鼠颞下颌关节骨关节机械痛阈值升高(P＜0.01),大鼠颞下颌关节液中IL-1β表达水平和颞下颌关节软骨Pelletier 评分以及软骨改良Mankin's评分降低(P＜0.01),大鼠颞下颌关节腔内注射医用臭氧 1 周各检测指标虽有变化但无显著性(P＞0.05).同一组内:医用臭氧组 3 周和 5 周相比于医用臭氧组 1 周,大鼠颞下颌关节机械痛阈值增加(P＜0.01),大鼠颞下颌关节液中IL-1β表达水平降低(P＜0.01),关节软骨Pelletier评分以及软骨改良Mankin's评分无统计学意义(P＞0.05).医用臭氧组3 周相比于医用臭氧组5 周,大鼠颞下颌关节机械痛阈值、大鼠颞下颌关节液中IL-1β表达水平、关节软骨Pelletier评分和软骨改良Mankin's评分无统计学意义(P＞0.05).结论 医用臭氧治疗 3 周以上可改善大鼠颞下颌关节骨关节炎及其疼痛.
Abstract
Objective To investigate the effect of medical ozone injection therapy on temporomandibular joint(TMJ)osteoarthritis and its pain in SD rats.Methods Fifity-four rats were randomly assigned according to a random number table into three groups:con-trol group,model group,and medical ozone group,with 18 rats in each group.In the control group,only physiological saline was in-jected during modeling;in the model group,only sodium iodoacetate was injected for modeling;in the medical ozone group,after in-jecting sodium iodoacetate into the joint cavity for modeling for one week,medical ozone was then injected into the joint cavity for inter-vention at a frequency of once a week,totaling 5 times.One week(week 2 after modeling),3 weeks(week 4 after modeling),and 5 weeks(week 6 after modeling)after medical ozone injections,6 rats from each group were euthanized.Mechanical withdrawal thresh-old of rats in each group was assessed before euthanasia,and the expression levels of interleukin-1β(IL-1β)in joint fluid of rats in each group were measured after euthanasia.Gross observation and modified Mankin's scoring were performed on TMJ cartilage of rats in each group after stained with Pelletier score and Safranin O-Fast Green.Results During the same time period,compared to the control group,the model group showed a significant decrease in the mechanical pain threshold of the TMJ in rats at 1 week,3 weeks,and 5 weeks(P＜0.01).The expression levels of IL-1β in the TMJ fluid increased(P＜0.01),and the Pelletier score and modified Mankin's score of TMJ cartilage increased(P＜0.01).In comparison to the model group,the medical ozone group exhibited a significant increase in the mechanical pain threshold of the TMJ in rats after 3 weeks and 5 weeks of medical ozone injections(P＜0.01).The expression levels of IL-1β in the TMJ fluid decreased(P＜0.01),and the Pelletier score and modified Mankin's score of TMJ cartilage decreased(P＜0.01).However,there were no statistically significant difference in the measured parameters in the TMJ cavity after 1 week of medical ozone injection(P＞0.05).Within the medical ozone group,compared to the 1-week treatment,the mechanical pain threshold of the TMJ increased(P＜0.01)and the expression levels of IL-1β in the TMJ fluid decreased at 3 weeks and 5 weeks(P＜0.01).However,there was no statistically significant difference in the Pelletier score and modified Mankin's score of TMJ cartilage(P＞0.05)between different treatment duration.Additionally,there were no statistically signif-icant differences in the mechanical pain threshold of the TMJ,expression levels of IL-1β in the TMJ fluid,Pelletier score,and modi-fied Mankin's score of TMJ cartilage between the medical ozone group at 3 weeks and 5 weeks(P＞0.05).Conclusion Medical ozone treatment for more than 3 weeks can improve temporomandibular joint osteoarthritis and its associated pain in rats.
NETL
NSTL
万方数据