The effect of NF-κB signaling pathway regulation on PD-L1 expression of Enterococcus faecalis LTA-stimulated macrophages
Objective To investigate the effect of Enterococcus faecalis(E.faecalis)lipoteichoic acid(LTA)on the expression of the macrophage immune checkpoint-programmed cell death protein ligand 1(PD-L1)and explore the possible regulatory mechanisms.Methods In vitro model was established by stimulating mouse monocyte macrophage cell line(RAW264.7)with different concentra-tions of E.faecalis LTA.PD-L1 expression and nuclear factor kappa-B(NF-κB)p65 phosphorylation level were detected using flow cy-tometry,quantitative real-time polymerase chain reaction(qRT-PCR),Western blot and cellular immunofluorescence.Macrophages were pretreated with the NF-κB signaling pathway inhibitor BAY 11-7082,then the expression of PD-L1 and p65 phosphorylation level were detected by the above methods.Results LTA induced PD-L1 expression in RAW264.7 cells,and p65 phosphorylation level in-creased(P＜0.05).Inhibition of the NF-κB signaling pathway could reduce p65 phosphorylation level,simultaneously reducing the ex-pression of PD-L1 on LTA-stimulated RAW264.7 cells(P＜0.05).Conclusion E.faecalis LTA can promote the PD-L1 expression of macrophages and this process is regulated by the NF-κB signaling pathway,which provides further insights into the occurrence and treatment of chronic periapical inflammation.
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