聚丙烯酸-柠檬酸盐-无定形磷酸钙的合成及其对牙本质再矿化的影响
Synthesis of polyacrylic acid-citrate-amorphous calcium phosphate and its effect on dentin remineralization
陈胤盈 1陈晨1
作者信息
- 1. 南京医科大学附属口腔医院牙体牙髓科,口腔疾病研究与防治国家级重点实验室培育建设点,江苏省口腔转化医学工程研究中心,江苏 南京(210029)
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摘要
目的 制备并表征聚丙烯酸-柠檬酸盐-无定形磷酸钙复合物(PAA-Cit-ACP),并分析其对牙本质的再矿化作用.方法 采用溶液共沉淀法及冷冻干燥法制备PAA-Cit-ACP,采用FTIR、XRD、Zeta电位、SEM、TEM及SAED对复合物进行表征.通过SEM分析PAA-Cit-ACP对牙本质再矿化的影响.通过CCK-8 法评估PAA-Cit-ACP的细胞毒性.结果 FTIR图谱中同时出现了羧基的特征峰(1 567 cm-1和 1 416 cm-1)和ACP的特征峰(1 064 cm-1和 563 cm-1),XRD及SAED结果共同证实了其无定形态,SEM及TEM图像均表现为球形纳米颗粒.PAA-Cit-ACP的Zeta电位值为-18.47 mV.牙本质SEM图示,经PAA-Cit-ACP处理后,脱矿牙本质表面形成了大量新的矿物质.CCK-8 结果示在 250、500、1 000 μg/mL浓度时,PAA-Cit-ACP均具有良好的生物相容性.结论 柠檬酸盐能与聚丙烯酸共同稳定过饱和钙磷溶液,形成聚丙烯酸-柠檬酸盐-无定形磷酸钙,并促进牙本质再矿化.
Abstract
Objective To prepare and identify the polyacrylic acid-citrate-amorphous calcium phosphate composite(PAA-Cit-ACP),and analyze its effect on dentin remineralization.Methods PAA-Cit-ACP was prepared by co-precipitation and freeze-drying methods.The composite was characterized by FTIR,XRD,Zeta potential,SEM,TEM and SAED.SEM was used to analyze the effect of PAA-Cit-ACP on dentin remineralization.Cell cytotoxicity of PAA-Cit-ACP was evaluated by CCK-8 method.Results The charac-teristic peaks of carboxyl(1 567 cm-1 and 1 416 cm-1)and ACP(1 064 cm-1 and 563 cm-1)were simultaneously observed in the FT-IR spectrum,and the XRD and SAED results confirmed its amorphous state.The Zeta potential of PAA-Cit-ACP was-18.47 mV.PAA-Cit-ACP appeared as spherical nanoparticles according to SEM and TEM.In SEM images,a large amount of new minerals formed on the surface of demineralized dentin after PAA-Cit-ACP treatment.The CCK-8 result showed that PAA-Cit-ACP exhibited good bio-compatibility at a concentration of 250,500,and 1 000 μg/mL.Conclusion Citrate can co-stabilize supersaturated calcium phosphate solutions with polyacrylic acid,forming polyacrylic acid citrate amorphous calcium phosphate which can promote dentin remi-neralization.
关键词
柠檬酸盐/无定形磷酸钙/牙本质再矿化Key words
citrate/amorphous calcium phosphate/dentin remineralization引用本文复制引用
出版年
2024
NETL
NSTL
万方数据