GsMTx4对大鼠牙齿移动过程中痛觉的抑制作用
The inhibitory effect of GsMTx4 on pain sensation during tooth movement in rats
王若飞 1邵丽鑫 1刘晓彤 1张苗苗1
作者信息
- 1. 哈尔滨医科大学附属第一医院正畸科,黑龙江 哈尔滨(150001)
- 折叠
摘要
目的 通过旷场实验、免疫组化染色,研究GsMTx4 在牙齿移动过程中对大鼠痛觉的抑制作用.方法 选取 58 只5～6 周雄性SD大鼠,并构建右上颌牙齿移动模型.随机将大鼠分为A组(对照组)、B组(GsMTx4 组)两组,另按加力时间4h和 1、3、5、7 d分为 5 个亚组,以A组大鼠左上颌作为C组(空白对照组).A、B两组大鼠分别局部注射生理盐水、GsMTx4.通过旷场实验评价大鼠的疼痛程度,免疫组化染色观察牙周组织中神经生长因子(nerve growth factor,NGF)、降钙素基因相关肽(calcitonin gene-related peptide,CGRP)的表达情况.结果 4 h、1 d、3 d时,B组大鼠在旷场中 5 min内的总路程较A组更高(P＜0.05).在 4h、1d时,B组大鼠中心区域停留时间百分比较A组高(P＜0.05).A、B两组中NGF、CGRP的表达在 1、3、5、7d时高于C组(P＜0.05).B组NGF、CGRP表达在 4 h、1 d、3 d的均低于A组(P＜0.05).结论 GsMTx4 在牙齿移动过程中能抑制NGF、CGRP的表达,并缓解牙齿移动时产生的疼痛.
Abstract
Objective To investigate the inhibitory effect of GsMTx4 on pain sensation in rats during tooth movement,open field ex-periments and immunohistochemical staining by conducting.Methods Fifty-eight male SD rats aged 5-6 weeks were selected and a right maxillary tooth movement model was constructed.The rats were randomly divided into two groups:group A(control group)and group B(GsMTx4 group).They were also divided into 5 subgroups according to 4 h,1 d,3 d,5 d,and 7 d.The left maxillary of the rats in group A was used as group C(blank control group).The open-field test was used to evaluate the pain level of rats.Immunohisto-chemical staining was used to observe the expression of nerve growth factor(NGF)and calcitonin gene-related peptide(CGRP)in peri-odontal tissues.Results At 4 h,1 d,and 3 d,the total distance traveled by rats in group B within 5 minutes in the open-field was higher than that of group A(P＜0.05).At 4 h and 1 d,the percentage of time in the center of rats in group B was higher than that in group A(P＜0.05).The expressions of NGF and CGRP in groups A and B were higher than those in group C on 1 d,3 d,5 d,and 7 d(P＜0.05).The expression of NGF and CGRP in group B were lower than those in group A at 4 h,1 d,and 3 d(P＜0.05).Conclu-sion GsMTx4 can inhibit the expression of NGF and CGRP during tooth movement and relieve the pain caused by tooth movement.
关键词
GsMTx4/正畸/疼痛/神经生长因子/降钙素基因相关肽Key words
GsMTx4/orthodontic/pain/NGF/CGRP引用本文复制引用
出版年
2024
NETL
NSTL
万方数据