Effects of Lemon Essential Oil on Acid Production and Activity of Lactic Dehydrogenase in Streptococcus Mutans
Objective:To investigate the effects of LEO on the acid production and activity of lactate dehydrogenase (LDH) in Streptococcus mutans,and to provide a theoretical basis for the application of lemon essential oil (LEO) in oral cavity.Methods:According to the minimal inhibitory concentration (MIC) of LEO,the dilution of 1/8MIC,1/4MIC,1/2MIC of LEO and the control group (only contained medium) were prepared by using two-fold dilution method.Streptococcus mutans was added into each group and cultivated for 24h.△pH of medium in each group was determined.The method of oxidation of reduction coellzyme Ⅰ and RT-PCR were respectively adopted to evaluate the activity of LDH and the expression of ldh gene related to acid production.Results:With increasing concentrations of LEO,a dose-dependent decline of△pH (1.423-0.067) and the activity of LDH (33.279-13.367 U/mg) was observed in the LEO group compared to the control group at sub-MIC levels (P＜0.01).Compared to the control group,the expression of ldh gene significantly decreased (P＜0.05).Conclusion:Sub-MICs of LEO inhibited the activity of LDH and reduced acid production by down-regulating the expression of ldh gene without affecting bacterial growth.
NETL
NSTL
万方数据
维普
