Objective:To investigate the effect of LIF during dentin formation.Methods:Immunohistochemistry(IHC)was employed to detect the expression pattern of LIF during odontoblast differentiation in PN2 incisors.A-bout 2mm from the tip of the erupted part of the left incisor of each mouse was removed to compare different growth speed between Lif group and the control group.The mandibles were scanned by a micro-computed tomography(Micro-CT)instrument.Mimics was used to analyze the mineral density of dentin in both groups.Microhardness measurement was carried out to measure the dentin surface hardness.Electron probe X-ray microanalyser(EPMA)was performed for surface elements analysis of mandibular incisors.Immunofluorescence(IF)was employed to de-tect the expression of Col1 α1 and DMP1 in odontoblasts.Results:LIF was present in polarizing odontoblasts,and gradually increased with the mature of odontoblasts.The growth speed of Lif--incisors were slower than that of control group.The mineral density and Vickers hardness of dentin in Lif-/-incisors were lower than that in wild-type mice.The element atomic percent of the Ca/P ratio was reduced in Lif-/-mice.Knockdown of Lif decreased the ALP activity and the formation of mineralized matrix nodules and these could be rescued by adding LIF.The ex-pressions of Col1 α1 and DMP1 were decreased in odontoblasts of Lif-/-group.Conclusion:Deficiency of LIF re-strains the formation and mineralization of incisor dentin in mice.
万方数据
维普
