Formononetin Motivates Osteogenic Differentiation of Dental Follicle Stem Cells
Objective:To elucidate the effect of formononetin on osteogenic differentiation of human dental follicle stem cells(hDFSCs).Methods:hDFSCs were isolated,cultured,and identified.The cells were treated with differ-ent concentrations of formononetin(0,0.1,1,and 10 μmol/L)in the culture medium.Cell viability was assessed by CCK-8 assay,crystal violet staining,and live/dead cell fluorescence staining.The cell cytoskeleton was exam-ined using fluorescent staining.The expression of osteogenic-related genes was analyzed through RT-qPCR.Alka-line phosphatase(ALP)activity was assessed to evaluate the osteogenic differentiation of cells.The influence of for-mononetin on calcium nodule formation during osteogenic differentiation was evaluated using alizarin red staining.Results:hDFSCs exhibited stem cell characteristics.Different concentrations of formononetin had no significant effect on hDFSCs viability.Whereas,1 μmol/L formononetin facillitated the expression of Runx2,OCN,and Col-Iα1 mRNA.Additionally,1 μmol/L formononetin enhanced the intracellular ALP activity and the number of calcium nodules.Conclusion:Formononetin at 1 μmol/L promotes the osteogenic differentiation of hDFSCs.
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维普
