Objective:To explore the potential of MYO5 A gene as a prognostic molecular marker in head and neck squamous cell carcinoma(HNSCC).Methods:RNA-Seq data and corresponding clinical data were downloaded from the TCGA database for the HNSCC queue,and the relationship between MYO5 A and patient prognosis was ana-lyzed using univariate Cox regression.The variable splicing types of the MYO5 A gene were downloaded from the TCGA SpliceSeq database,the variable splicing of the differentially expressed MYO5 A gene was identified based on the percentage spliced in(PSI)value,and its correlation with the prognosis of HNSCC patients was analyzed.Fi-nally,RT-PCR and Sanger sequencing were validated in clinical samples from cell lines and affiliated hospitals of Xuzhou Medical University.Results:The MYO5 A gene was significantly overexpressed in the HNSCC cohort,but the overall expression level of the MYO5 A gene could not be used as an independent prognostic risk indicator for HNSCC.The frequency of MYO5 A exon 35 hopping was increased in HNSCC and was closely related to prognosis.The jump of MYO5 A exon 35 was validated in both cell lines and clinical samples.Conclusion:The jump of MYO5 A exon 35 rather than the overall expression level of MYO5 A gene can serve as an independent molecular marker for the prognosis of HNSCC.
关键词
MYO5A/头颈鳞状细胞癌/可变剪接/预后
Key words
MYO5 A/head and neck squamous cell carcinoma/alternative splicing/prognosis
万方数据