Prognostic Correlation Study of MYO5 A Exon 35 Skipping in Head and Neck Squamous Cell Carcinoma
Objective:To explore the potential of MYO5 A gene as a prognostic molecular marker in head and neck squamous cell carcinoma(HNSCC).Methods:RNA-Seq data and corresponding clinical data were downloaded from the TCGA database for the HNSCC queue,and the relationship between MYO5 A and patient prognosis was ana-lyzed using univariate Cox regression.The variable splicing types of the MYO5 A gene were downloaded from the TCGA SpliceSeq database,the variable splicing of the differentially expressed MYO5 A gene was identified based on the percentage spliced in(PSI)value,and its correlation with the prognosis of HNSCC patients was analyzed.Fi-nally,RT-PCR and Sanger sequencing were validated in clinical samples from cell lines and affiliated hospitals of Xuzhou Medical University.Results:The MYO5 A gene was significantly overexpressed in the HNSCC cohort,but the overall expression level of the MYO5 A gene could not be used as an independent prognostic risk indicator for HNSCC.The frequency of MYO5 A exon 35 hopping was increased in HNSCC and was closely related to prognosis.The jump of MYO5 A exon 35 was validated in both cell lines and clinical samples.Conclusion:The jump of MYO5 A exon 35 rather than the overall expression level of MYO5 A gene can serve as an independent molecular marker for the prognosis of HNSCC.
MYO5 Ahead and neck squamous cell carcinomaalternative splicingprognosis
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