Effects of Different Osteogenic Induction Systems on Osteogenic Differentiation of MC3T3-E1 Cells
Objective:To determine the optimal osteogenic induction system in vitro for MC3T3-E1 osteoblasts.Methods:Different osteogenic induction systems were constructed,which were dexamethasone 0.1 µmol/L,ascor-bic acid 50 μg/mL,and β-sodium glycerophosphate 10 mmol/L in group A;dexamethasone 0.01 μmol/L,ascorbic acid 50 µg/mL,and β-sodium glycerophosphate 10 mmol/L in group B;dexamethasone 1 µmol/L,ascorbic acid 50 μg/mL,and β-sodium glycerophosphate 10 mmol/L in group C;ascorbic acid 50 μg/mL and β-sodium glycero-phosphate 10 mmol/L in Group D;and ascorbic acid 100 µg/mL and β-sodium glycerophosphate 5 mmol/L in Group E.MC3T3-E1 cells were induced in vitro for 7 days and 14 days.The effects of alkaline phosphatase staining and alizarin red staining on osteogenic differentiation of MC3T3-E1 were analyzed.The expression levels of osteo-genic differentiation related genes(ALP,Runx-2,COL-1,OCN,OPN)were detected by RT-qPCR.Results:There was a significant difference in the optimal quantification of alizarin among groups B,D,and E(P＜0.05).The PCR results at 7 days after induction showed that the gene expression levels of ALP,Runx-2,and COL-1 in group C were the highest(P＜0.05).PCR results at 14 days after induction showed that the expression of OCN in Group D was the highest(P＜0.05).Conclusion:The con-centrations of dexamethasone and ascorbic acid have an effect on the osteogenic differentiation of MC3T3-E1 cells.Group C was better for early osteogenesis and Group D was better for late osteogenesis.However,in comprehensive considera-tion,Group B is recommended for late osteogenesis.
MC3T3-E1osteogenic differentiationinducement system
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