Ipriflavone Promotes Proliferation and Mineralization of Human Dental Pulp Stem Cells
Objective:To investigate the effects of ipriflavone(IP)on proliferation and mineralization of human dental pulp stem cells(hDPSCs).Methods:The hDPSCs were cultured in complete culture medium containing IP(10-9-10-5 mol/L)and identified.The cell activity at different time points(1,2,3 d)was detected by CCK-8.After induced for 7 days with mineralization liquid containing IP(10-8-10-5 mol/L),the alkaline phosphatase(ALP)activity,ALP staining,alizarin red staining,and RT-qPCR were used to detect the osteogenic differentiation of hDPSCs.Results:CCK-8 detection showed that 10-9-10-5 mol/L IP could promote the proliferation of hDPSCs,and 10-6 mol/L IP had the best results(P＜0.05).In 10-6 mol/L IP group,the ALP staining was deepened,the activity was increased(P＜0.05),and the mineralization nodules were increased.RT-qPCR showed that the con-tents of Runt-related transcription factor,ALP,and osteocalcin in the 10-6 mol/L IP group were significantly up-regulated(P＜0.05).Conclusion:10-6 mol/L IP can promote the proliferation and mineralization of hDPSCs.
dental pulp stem cellsmultiplicationdifferentiation of boneipriflavone
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