依普黄酮促进人牙髓干细胞增殖和成骨分化
Ipriflavone Promotes Proliferation and Mineralization of Human Dental Pulp Stem Cells
乐曼妮 1王小聪 1黄子璇 1张慧琳 1张晓月 1赵卿 1李明 1王基栋2
作者信息
- 1. 湖南中医药大学口腔医(学)院 湖南长沙 410208;长沙市口腔医院 湖南长沙 410004
- 2. 中南大学湘雅医学院附属常德医院 湖南常德 415000
- 折叠
摘要
目的:初步检测依普黄酮(1P)对人牙髓干细胞(human dental pulp stem cells,hDPSCs)的增殖和成骨向分化的影响.方法:在体外对hDPSCs进行培养鉴定,用含IP(10-9～10-5 mol/L)的完全培养液培养hDPSCs,CCK-8法检测不同时间点(1、2、3 d)的细胞活性;用含IP(10-8～10-5 mol/L)的矿化诱导液诱导hDPSCs 7 d,通过碱性磷酸酶活性测定、ALP染色、茜素红染色及RT-qPCR检测IP对hDPSCs成骨分化的影响.结果:CCK-8检测结果表明10-9～10-5 mol/L IP均可促进hDPSCs增殖,其中10-6 mol/L IP组促进增殖效果最佳(P＜0.05);10-6 mol/L IP组ALP染色加深,ALP活性增高(P＜0.05),矿化结节增多(P＜0.05);RT-qPCR检测结果显示10-6 mol/L IP组能够提高成骨分化相关基因骨钙素(osteocalcin,OCN)、碱性磷酸酶和矮小相关转录基因2的表达水平(P＜0.05).结论:10-6 mol/L IP能提高hDPSCs增殖和成骨向分化的能力.
Abstract
Objective:To investigate the effects of ipriflavone(IP)on proliferation and mineralization of human dental pulp stem cells(hDPSCs).Methods:The hDPSCs were cultured in complete culture medium containing IP(10-9-10-5 mol/L)and identified.The cell activity at different time points(1,2,3 d)was detected by CCK-8.After induced for 7 days with mineralization liquid containing IP(10-8-10-5 mol/L),the alkaline phosphatase(ALP)activity,ALP staining,alizarin red staining,and RT-qPCR were used to detect the osteogenic differentiation of hDPSCs.Results:CCK-8 detection showed that 10-9-10-5 mol/L IP could promote the proliferation of hDPSCs,and 10-6 mol/L IP had the best results(P＜0.05).In 10-6 mol/L IP group,the ALP staining was deepened,the activity was increased(P＜0.05),and the mineralization nodules were increased.RT-qPCR showed that the con-tents of Runt-related transcription factor,ALP,and osteocalcin in the 10-6 mol/L IP group were significantly up-regulated(P＜0.05).Conclusion:10-6 mol/L IP can promote the proliferation and mineralization of hDPSCs.
关键词
牙髓干细胞/增殖/成骨分化/依普黄酮Key words
dental pulp stem cells/multiplication/differentiation of bone/ipriflavone引用本文复制引用
基金项目
湖南省自然科学基金面上项目(2022JJ30630)
湖南省科技厅临床医疗技术创新引导项目(2021SK53301)
湖南省卫生健康委重点指导课题(202108051626)
湖南省教育厅重点项目(2020SK2137)
长沙市自然科学基金(kq2208458)
出版年
2024
NETL
NSTL
万方数据