目的:探讨mTOR调节相关蛋白(regulatory-associated protein of mTOR,RAPTOR)对口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)迁移、侵袭和增殖能力的影响.方法:利用TCGA生物信息数据库查询在头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)组织与癌旁组织中差异表达的mRNA.West-ern blot检测RAPTOR在人口腔上皮细胞HOEC和OSCC细胞系中的表达.利用伤口愈合实验、Transwell实验、EdU实验检测各组细胞迁移、侵袭及增殖能力.生物信息学网站预测与RAPTOR靶向结合的微小RNA(mi-croRNA,miR).功能学实验验证miR-485-5p是否可以靶向RAPTOR影响OSCC细胞的迁移、侵袭和增殖.结果:RAPTOR在HNSCC组织中较癌旁组织表达增高.伤口愈合、Transwell和EdU实验结果示,RAPTOR有促进OSCC细胞的迁移、侵袭和增殖能力.miR-485-5p能与RAPTOR靶向结合,且miR-485-5p上调能逆转RAPTOR促进CAL27细胞迁移、侵袭和增殖能力.结论:miR-485-5p通过靶向RAPTOR抑制OSCC细胞迁移、侵袭和增殖能力.
RAPTOR Affects Migration,Invasion,and Proliferation of Oral Squamous Cell Carcinoma through miR-485-5p
Objective:To investigate the effect of regulatory-associated protein of mTOR(RAPTOR)on the mi-gration,invasion,and proliferation of oral squamous cell carcinoma(OSCC).Methods:The TCGA database was used to identify differentially expressed mRNA in head and neck squamous cell carcinoma(HNSCC)tissues and ad-jacent tissues.Western blot assay detected RAPTOR expression in HOEC and OSCC cell lines.Cell migration abili-ty was detected by the wound healing experiment.Trans well assay and EdU were used to detect the cell invasion and cell proliferation ability.Bioinformatics websites were used to predict microRNA binding to RAPTOR.Func-tional tests were conducted to determine if miR-485-5p could impact cell migration,invasion,and proliferation by targeting RAPTOR.Results:RAPTOR expression was higher in HNSCC tissues compared to adjacent tissues.Wound healing,Transwell,and EdU experiments showed that RAPTOR promoted the migration,invasion,and proliferation of OSCC cells.Up-regulation of miR-485-5p can reverse the promoting effect of RAPTOR on the mi-gration,invasion,and proliferation of CAL27 cells.Conclusion:miR-485-5p inhibits OSCC cell migration,inva-sion,and proliferation by targeting RAPTOR.
万方数据
维普
